| Encephalitic listeriosis in ruminants: immunohistochemistry as a diagnostic tool. | |
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MedLine Citation:
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PMID: 15610491 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A retrospective analysis of 42 ruminants (sheep, goats and cattle) with suspected meningo-encephalitis was performed. The clinical findings and the post-mortem results of the animals have been specified. Bacteriological culture, Gram's stain and Listeria-specific immunohistochemistry were performed in order to confirm the diagnosis of these cases. The results of the different methods were evaluated for the detection of listerial antigens. Bacteriological culture was positive in 28.5% of the cases. In 47.6% of the cases, Gram-positive bacteria were found and 80.9% of the cases were immunohistochemically positive for the listerial antigen. The most important conclusion from this investigation is that the traditionally used Gram's stain and the bacteriological culture techniques are insufficient compared with immunohistochemistry for the confirmation of a Listeria monocytogenes infection. |
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Authors:
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E Loeb |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of veterinary medicine. A, Physiology, pathology, clinical medicine Volume: 51 ISSN: 0931-184X ISO Abbreviation: J Vet Med A Physiol Pathol Clin Med Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-12-21 Completed Date: 2005-05-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100955112 Medline TA: J Vet Med A Physiol Pathol Clin Med Country: Germany |
Other Details:
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Languages: eng Pagination: 453-5 Citation Subset: IM |
Affiliation:
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Faculty of Veterinary Medicine, Department of Pathobiology, Utrecht University, PO Box 80158, NL-3508 TD, Utrecht, The Netherlands. e.loeb@vet.uu.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Bacterial / isolation & purification* Cattle Cattle Diseases / diagnosis*, microbiology Encephalitis / diagnosis, microbiology, veterinary* Goat Diseases / diagnosis*, microbiology Goats Immunohistochemistry / veterinary Listeria / immunology, isolation & purification Listeria Infections / diagnosis, veterinary* Retrospective Studies Sheep Sheep Diseases / diagnosis*, microbiology Staining and Labeling / veterinary |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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