Document Detail


Encephalitic listeriosis in ruminants: immunohistochemistry as a diagnostic tool.
MedLine Citation:
PMID:  15610491     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A retrospective analysis of 42 ruminants (sheep, goats and cattle) with suspected meningo-encephalitis was performed. The clinical findings and the post-mortem results of the animals have been specified. Bacteriological culture, Gram's stain and Listeria-specific immunohistochemistry were performed in order to confirm the diagnosis of these cases. The results of the different methods were evaluated for the detection of listerial antigens. Bacteriological culture was positive in 28.5% of the cases. In 47.6% of the cases, Gram-positive bacteria were found and 80.9% of the cases were immunohistochemically positive for the listerial antigen. The most important conclusion from this investigation is that the traditionally used Gram's stain and the bacteriological culture techniques are insufficient compared with immunohistochemistry for the confirmation of a Listeria monocytogenes infection.
Authors:
E Loeb
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of veterinary medicine. A, Physiology, pathology, clinical medicine     Volume:  51     ISSN:  0931-184X     ISO Abbreviation:  J Vet Med A Physiol Pathol Clin Med     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-21     Completed Date:  2005-05-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955112     Medline TA:  J Vet Med A Physiol Pathol Clin Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  453-5     Citation Subset:  IM    
Affiliation:
Faculty of Veterinary Medicine, Department of Pathobiology, Utrecht University, PO Box 80158, NL-3508 TD, Utrecht, The Netherlands. e.loeb@vet.uu.nl
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Bacterial / isolation & purification*
Cattle
Cattle Diseases / diagnosis*,  microbiology
Encephalitis / diagnosis,  microbiology,  veterinary*
Goat Diseases / diagnosis*,  microbiology
Goats
Immunohistochemistry / veterinary
Listeria / immunology,  isolation & purification
Listeria Infections / diagnosis,  veterinary*
Retrospective Studies
Sheep
Sheep Diseases / diagnosis*,  microbiology
Staining and Labeling / veterinary
Chemical
Reg. No./Substance:
0/Antigens, Bacterial

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