Document Detail


Encephalic photoreception and phototactic response in the troglobiont Somalian blind cavefish Phreatichthys andruzzii.
MedLine Citation:
PMID:  22837464     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many physiological and behavioural responses to changes in environmental lighting conditions are mediated by extraocular photoreceptors. Here we investigate encephalic photoreception in Phreatichthys andruzzii, a typical cave-dwelling fish showing an extreme phenotype with complete anophthalmy and a reduction in size of associated brain structures. We firstly identified two P. andruzzii photopigments, orthologues of rod opsin and exo-rod opsin. In vitro, both opsins serve as light-absorbing photopigments with λ(max) around 500 nm when reconstituted with an A(1) chromophore. When corrected for the summed absorption from the skin and skull, the spectral sensitivity profiles shifted to longer wavelengths (rod opsin: 521 nm; exo-rod opsin: 520 nm). We next explored the involvement of both opsins in the negative phototaxis reported for this species. A comparison of the spectral sensitivity of the photophobic response with the putative A(2) absorbance spectra corrected for skin/skull absorbance indicates that the A(2) versions of either or both of these pigments could explain the observed behavioural spectral sensitivity.
Authors:
Emma E Tarttelin; Elena Frigato; James Bellingham; Viviana Di Rosa; Roberto Berti; Nicholas S Foulkes; Robert J Lucas; Cristiano Bertolucci
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  215     ISSN:  1477-9145     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-27     Completed Date:  2012-11-28     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2898-903     Citation Subset:  IM    
Affiliation:
Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Amino Acid Sequence
Animals
Blindness / physiopathology*
Brain / metabolism*
Caves
Cypriniformes / physiology*
HEK293 Cells
Humans
Light Signal Transduction / physiology*
Molecular Sequence Data
Photobleaching
Photoreceptor Cells, Vertebrate / metabolism*
Rod Opsins / chemistry,  metabolism
Sequence Alignment
Somalia
Spectrum Analysis
Time Factors
Grant Support
ID/Acronym/Agency:
WT078808//Wellcome Trust
Chemical
Reg. No./Substance:
0/Rod Opsins
Comments/Corrections

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