Document Detail


Encapsulation of rhodanese and organic thiosulfonates by mouse erythrocytes.
MedLine Citation:
PMID:  7958565     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A series of organic thiosulfonates were synthesized and studied as sulfur donor substrates for rhodanese encapsulated within murine carrier erythrocytes. Previous studies have indicated that resealed erythrocytes containing rhodanese (CRBC) and sodium thiosulfate can rapidly metabolize cyanide to the less toxic thiocyanate. This thiosulfate-rhodanese system was very efficacious as a new conceptual approach to antagonize cyanide intoxication both in vitro and in vivo. However, its potential is restricted because of the limited availability of thiosulfate due to its poor permeability through RBC membrane. Present studies suggest that there are advantages in using alternative sulfur donors, i.e., organic thiosulfonates in this rhodanese-containing resealed erythrocyte system, since these compounds have higher lipid solubility than inorganic thiosulfates and can readily penetrate the red blood cell membrane. Therefore, this system could provide a virtually unlimited amount of sulfur donor to the encapsulated rhodanese even if the substrates are in solution outside the cells. Moreover, the rhodanese reaction rate of any of these organic thiosulfonates is much faster than the rate observed with the classic cyanide antidote, sodium thiosulfate. This CRBC system will continue to detoxify cyanide even when these encapsulated sulfur donors are depleted, as the lipid soluble organic thiosulfonate outside the cells will diffuse past the membrane into the cell to replenish the sulfur donor. The encapsulation efficiency for rhodanese is about 30%, and the velocity of the rhodanese reaction increases linearly with the volume of enzyme-laden erythrocytes. Similarly, reaction velocity increases linearly with substrate concentration.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
I Petrikovics; L Pei; W D McGuinn; E P Cannon; J L Way
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Fundamental and applied toxicology : official journal of the Society of Toxicology     Volume:  23     ISSN:  0272-0590     ISO Abbreviation:  Fundam Appl Toxicol     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-12-01     Completed Date:  1994-12-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8200838     Medline TA:  Fundam Appl Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  70-5     Citation Subset:  IM    
Affiliation:
Department of Medical Pharmacology and Toxicology, Texas A&M University, College of Medicine, College Station 77843-1114.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cyanides / antagonists & inhibitors
Erythrocytes / enzymology,  metabolism*
Male
Mice
Mice, Inbred BALB C
Sulfur / metabolism
Thiosulfate Sulfurtransferase / metabolism*
Thiosulfonic Acids / metabolism*
Chemical
Reg. No./Substance:
0/Cyanides; 0/Thiosulfonic Acids; 0/butanethiosulfonic acid; 7704-34-9/Sulfur; EC 2.8.1.1/Thiosulfate Sulfurtransferase

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