Document Detail


Encapsulation of lactic acid bacteria in colloidosomes.
MedLine Citation:
PMID:  23113619     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Polymeric colloidosomes encapsulating viable lactic acid bacteria were prepared. An aqueous suspension of 153 nm poly(methyl methacrylate-co-butylacrylate) latex particles plus Lactobacillus crispatus was emulsified in a continuous phase of sunflower oil. By adding a small amount of ethanol and salt to the oil phase the latex particles at the surface of the emulsion droplets aggregate to form the colloidosome shells. The colloidosomes have been examined using optical, confocal and scanning electron microscopies. The viability of the bacteria was tested using fluorescent molecular probes. The encapsulated lactic acid bacteria were able to metabolize glucose from solution and produce acid albeit at a slower rate compared to unencapsulated microbes. This demonstrates transport limitation through the colloidosome shell and restriction of the cellular metabolism due to encapsulation. Protection of the bacteria by encapsulation in colloidosomes was also demonstrated; a significantly larger number of encapsulated bacteria maintained viability in simulated stomach conditions compared to unencapsulated microbes.
Authors:
Polly Hr Keen; Nigel K H Slater; Alexander Francis Routh
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-31
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  -     ISSN:  1520-5827     ISO Abbreviation:  Langmuir     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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