Document Detail


Encapsulation, in vitro characterization, and in vivo biocompatibility of Sertoli cells in alginate-based microcapsules.
MedLine Citation:
PMID:  17335402     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for microencapsulation of isolated neonatal porcine Sertoli cells is described. Using a conventional alginate-poli-L-ornithine encapsulation procedure, which has been used in our laboratory for almost two decades to envelop pancreatic islets, we observed significant loss of Sertoli cell viability, possibly due to excessive Ca(2+) ion exposure. Replacing calcium with barium, or shortening the incubation period in the presence of Ca ions, we obtained barium or calcium alginate gel microbeads that did not alter morphology and viability of the encapsulated Sertoli cells. The procedure might permit access to a novel approach to immunologically alter cell graft acceptance.
Authors:
Giovanni Luca; Mario Calvitti; Claudio Nastruzzi; Luca Bilancetti; Ennio Becchetti; Gabriella Angeletti; Francesca Mancuso; Riccardo Calafiore
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering     Volume:  13     ISSN:  1076-3279     ISO Abbreviation:  Tissue Eng.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-16     Completed Date:  2007-06-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9505538     Medline TA:  Tissue Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  641-8     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Section of Internal Medicine and Endocrine and Metabolic Sciences, University of Perugia, Perugia, Italy.
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MeSH Terms
Descriptor/Qualifier:
Alginates*
Animals
Biocompatible Materials*
Capsules
Cells, Cultured
Glucuronic Acid
Hexuronic Acids
Insulin-Like Growth Factor I / secretion
Male
Mice
Mice, Inbred NOD
Microscopy, Electron, Transmission
Sertoli Cells* / secretion,  transplantation,  ultrastructure
Swine
Chemical
Reg. No./Substance:
0/Alginates; 0/Biocompatible Materials; 0/Capsules; 0/Hexuronic Acids; 576-37-4/Glucuronic Acid; 67763-96-6/Insulin-Like Growth Factor I; 9005-32-7/alginic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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