Document Detail


Encapsulation of the cobra cytotoxin P4 in liposomes.
MedLine Citation:
PMID:  8439402     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cytotoxin P4 isolated from the venom of the cobra Naja nigricollis nigricollis was encapsulated in liposomes by the reverse-phase evaporation method using phosphatidylcholine, cholesterol and phosphatidylethanolamine at molar ratios of 3:1:1. Cytotoxicity examination on murine melanoma B16F10 revealed that the activity of the entrapped cytotoxin did not change significantly and remained stable for at least 1 year at 4 degrees C without any significant leak from the liposomes (< 1%). Moreover, the activity of the cytotoxic liposomes was 20-fold higher towards human erythrocytes. Whereas 45 micrograms of the free cytotoxin/ml were needed for total haemolysis, the cytotoxic liposomes brought about the same effect at cytotoxin concentrations of 2-2.5 micrograms/ml, indicating the potential of the liposomes as a delivery system for the snake toxin.
Authors:
A Chaim-Matyas; G Borkow; M Ovadia
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  17 ( Pt 1)     ISSN:  0885-4513     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-03-30     Completed Date:  1993-03-30     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  31-6     Citation Subset:  IM    
Affiliation:
Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Death
Cobra Venoms / chemistry,  toxicity*
Drug Carriers
Drug Compounding
Erythrocytes / drug effects
Hemolysis / drug effects
Humans
Liposomes*
Melanoma, Experimental
Mice
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Cobra Venoms; 0/Drug Carriers; 0/Liposomes; 0/cytotoxin P4

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