| Encapsulation of the cobra cytotoxin P4 in liposomes. | |
| | |
MedLine Citation:
|
PMID: 8439402 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cytotoxin P4 isolated from the venom of the cobra Naja nigricollis nigricollis was encapsulated in liposomes by the reverse-phase evaporation method using phosphatidylcholine, cholesterol and phosphatidylethanolamine at molar ratios of 3:1:1. Cytotoxicity examination on murine melanoma B16F10 revealed that the activity of the entrapped cytotoxin did not change significantly and remained stable for at least 1 year at 4 degrees C without any significant leak from the liposomes (< 1%). Moreover, the activity of the cytotoxic liposomes was 20-fold higher towards human erythrocytes. Whereas 45 micrograms of the free cytotoxin/ml were needed for total haemolysis, the cytotoxic liposomes brought about the same effect at cytotoxin concentrations of 2-2.5 micrograms/ml, indicating the potential of the liposomes as a delivery system for the snake toxin. |
| | |
Authors:
|
A Chaim-Matyas; G Borkow; M Ovadia |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biotechnology and applied biochemistry Volume: 17 ( Pt 1) ISSN: 0885-4513 ISO Abbreviation: Biotechnol. Appl. Biochem. Publication Date: 1993 Feb |
Date Detail:
|
Created Date: 1993-03-30 Completed Date: 1993-03-30 Revised Date: 2007-03-21 |
Medline Journal Info:
|
Nlm Unique ID: 8609465 Medline TA: Biotechnol Appl Biochem Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 31-6 Citation Subset: IM |
Affiliation:
|
Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Death Cobra Venoms / chemistry, toxicity* Drug Carriers Drug Compounding Erythrocytes / drug effects Hemolysis / drug effects Humans Liposomes* Melanoma, Experimental Mice Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Cobra Venoms; 0/Drug Carriers; 0/Liposomes; 0/cytotoxin P4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of collagen cross-linking techniques for the stabilization of tissue matrices.
Next Document: Sugar-coated liposomes: a novel delivery system for increased drug efficacy and reduced drug toxicit...