| Encapsulation of Pseudomonas sp. ADP cells in electrospun microtubes for atrazine bioremediation. | |
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MedLine Citation:
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PMID: 22760669 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Electrospun hollow polymeric microfibers (microtubes) were evaluated as an encapsulation method for the atrazine degrading bacterium Pseudomonas sp. ADP. Pseudomonas sp. ADP cells were successfully incorporated in a formulation containing a core solution of polyethylene oxide dissolved in water and spun with an outer shell solution made of polycaprolactone and polyethylene glycol dissolved in a chloroform and dimethylformamide. The resulting microtubes, collected as mats, were partially collapsed with a ribbon-like structure. Following encapsulation, the atrazine degradation rate was low (0.03 ± 0.01 mg atrazine/h/g fiber) indicating that the electrospinning process negatively affected cell activity. Atrazine degradation was restored to 0.5 ± 0.1 mg atrazine/h/g fiber by subjecting the microtubes to a period of growth. After 3 and 7 days growth periods, encapsulated cells were able to remove 20.6 ± 3 and 47.6 ± 5.9 mg atrazine/g mat, respectively, in successive batches under non-growth conditions (with no additional electron donor) until atrazine was detected in the medium. The loss of atrazine degrading capacity was regained following an additional cell-growth period. |
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Authors:
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Shiri Klein; Ron Avrahami; Eyal Zussman; Michael Beliavski; Sheldon Tarre; Michal Green |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-4 |
Journal Detail:
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Title: Journal of industrial microbiology & biotechnology Volume: - ISSN: 1476-5535 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9705544 Medline TA: J Ind Microbiol Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Faculty of Civil and Environmental Engineering, Technion, Israel Institute of Technology, 32000, Haifa, Israel, shirikl@wildblue.net. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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