Document Detail

Enantiospecific assay of citadiol--a key intermediate of escitalopram by liquid chromatography on Chiralpak AD-H column connected with UV and polarimetric detectors in series.
MedLine Citation:
PMID:  16859854     Owner:  NLM     Status:  MEDLINE    
A simple, rapid, selective and reproducible LC method for separation and quantitative determination of citadiol (CTD), a key intermediate of escitalopram has been developed. An optimum resolution > 3.0 was achieved on Chiralpak AD-H (250 mm x 4.6 mm); 5 microm column connected with UV and polarimetric detectors in series. The effects of organic modifiers, viz., methanol, ethanol, n-propanol and 2-propanol on enantioselectivity were evaluated. The limits of detection (LOD) and quantification (LOQ) were 0.02 microg/ml, 0.03 microg/ml and 0.07 microg/ml, 0.10 microg/ml for R-CTD and S-CTD enantiomers, respectively. The linearity of the method was studied in the range of 0.07-300 microg/ml and 0.1-300 microg/ml for R-CTD and S-CTD, respectively and the r2 was > or = 0.9999. The inter- and intra-day assay precision was less than 0.74% (%R.S.D.) and the recoveries were in the range 99.68-100.72% with %R.S.D. < 0.49%.
R Nageswara Rao; A Narasa Raju
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Publication Detail:
Type:  Journal Article     Date:  2006-07-21
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  43     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-12     Completed Date:  2007-03-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  311-4     Citation Subset:  IM    
HPLC/UV Group, Division of Analytical Chemistry, Discovery Laboratory, Indian Institute of Chemical Technology, Hyderabad 500007, India.
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MeSH Terms
Chromatography, High Pressure Liquid
Citalopram / analysis*
Indicators and Reagents
Nitriles / analysis*
Polysaccharides / analysis
Reference Standards
Reproducibility of Results
Spectrophotometry, Ultraviolet
Reg. No./Substance:
0/Indicators and Reagents; 0/Nitriles; 0/Polysaccharides; 0/citadiol; 59729-33-8/Citalopram

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