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Enantioseparation of organic acids of pharmaceutical interest using eremomycin as a chiral selector.
MedLine Citation:
PMID:  21905045     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Strong adsorption of eremomycin on the fused-silica capillary wall was used for separation of enantiomers by CE. The capillary with adsorbed chiral selector was shown to be easily prepared and has reproducible properties. The effect of the chiral selector concentration, pH and composition of the BGE, and applied voltage on enantioseparation of acidic compounds, such as profens and aromatic carboxylic acids, was investigated. Two native α-amino acids, aspartic acid and glutamic acid, were enantioseparated. Fourteen tested compounds (including amino acids) were baseline resolved. Good selectivity of separation (α>1.09) was achieved. The migration order of ibuprofen and ketoprofen enantiomers was determined. The procedures were proposed for the analysis of flurbiprofen and warfarin in pharmaceuticals. Linearity was achieved in the concentration range of 4.0×10(-5) -2.0×10(-3)  M for flurbiprofen and 3.2×10(-6) -4.9×10(-6)  M for warfarin. The detection limits were found to be about 1×10(-5)  M for flurbiprofen and 1×10(-6)  M for warfarin.
Authors:
Aleksandra F Prokhorova; Vladimir A Larin; Anna N Mikhalyuk; Sergei M Staroverov; Elena N Shapovalova; Oleg A Shpigun
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-8
Journal Detail:
Title:  Electrophoresis     Volume:  -     ISSN:  1522-2683     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Division of Analytical Chemistry, Chemistry Department, Lomonosov Moscow State University, Leninskie Gory, Moscow, Russia. alexapro@gmail.com.
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