Document Detail

Enantioseparation of amino acids and dipeptides using vancomycin as chiral selector in capillary electrophoresis.
MedLine Citation:
PMID:  9034779     Owner:  NLM     Status:  MEDLINE    
Vancomycin was applied as chiral selector for the enantiomeric separation of derivatized amino acids and dipeptides. The influence of vancomycin concentration, pH and presence of 2-propanol in the buffer were examined in order to find optimal separation conditions. Optimization was by factorial design. Further, chiral separation of derivatives prepared with three different reagents was compared. These reagents were 9-fluorenylmethyl chloroformate (FMOC), 2-(9-anthryl)ethyl chloroformate (AEOC) and dansyl chloride (dansyl). Optimum resolution was at high vancomycin concentrations, while optimum efficiency was at low vancomycin concentrations. As a consequence of the very high enantioselectivity of vancomycin, the vancomycin concentration below the amount necessary for maximal resolution can be used. Separation efficiency was relatively low, and this could be attributed to adsorption of the selector at the capillary wall. Three factors led to decreased adsorption: application of a pH above the zero mobility pH value, low vancomycin concentrations and the presence of 2-propanol. For amino acids, the resolutions of the different derivatives were: dansyl > AEOC > FMOC, while for dipeptides, the highest selectivity was with AEOC.
H Wan; L G Blomberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  17     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-09-02     Completed Date:  1997-09-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  1938-44     Citation Subset:  IM    
Department of Analytical Chemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden.
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MeSH Terms
Amino Acids / isolation & purification*
Dansyl Compounds
Dipeptides / isolation & purification*
Electrophoresis, Capillary / methods*
Formic Acid Esters
Hydrogen-Ion Concentration
Indicators and Reagents
Reg. No./Substance:
0/2-(9-anthryl)ethyl chloroformate; 0/Amino Acids; 0/Anthracenes; 0/Dansyl Compounds; 0/Dipeptides; 0/Fluorenes; 0/Formic Acid Esters; 0/Indicators and Reagents; 1404-90-6/Vancomycin; 28920-43-6/1-(9-fluorenyl)methyl chloroformate; 605-65-2/dansyl chloride; 71-23-8/1-Propanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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