| Enantioseparation of amino acids, alpha-hydroxy acids, and dipeptides by ligand-exchange CEC using silica-based chiral stationary phases. | |
| | |
MedLine Citation:
|
PMID: 19655330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This work deals with the application of silica-based ligand-exchange chiral stationary phases (CSPs) for the enantioseparation of underivatized amino acids, alpha-hydroxy acids, and dipeptides with packed CEC. Two different possibilities of preparing silica-based CSPs are presented. One phase contains L-4-hydroxyproline chemically bonded via a spacer to 3 mum silica material. The other approach makes use of N-decyl-L-4-hydroxyproline dynamically coated on a reversed-phase packed capillary. Dynamical coating of reversed-phase material represents a simple alternative to prepare CSP. A comparison of the chemically bonded phase with the dynamically coated CSP by means of resolution of complex-forming analytes is presented. The chemically bonded phase was found to be superior to the dynamically coated phase in terms of resolution of amino acids and dipeptides. However, the dynamically coated CSP was found to be especially suitable for the separation of alpha-hydroxy acids. Both techniques are applicable for enantiomer purity tests. |
| | |
Authors:
|
Elfriede Pittler; Nina Grawatsch; D??rte Paul; Gerald G??bitz; Martin G Schmid |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Electrophoresis Volume: 30 ISSN: 1522-2683 ISO Abbreviation: Electrophoresis Publication Date: 2009 Aug |
Date Detail:
|
Created Date: 2009-08-26 Completed Date: 2010-01-05 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
|
Languages: eng Pagination: 2897-904 Citation Subset: IM |
Affiliation:
|
Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Karl-Franzens-University, Austria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
isolation & purification* Capillary Electrochromatography / methods* Dipeptides / isolation & purification* Hydrogen-Ion Concentration Hydroxy Acids / isolation & purification* Ligands Phenylalanine / analogs & derivatives, isolation & purification Silicon Dioxide / chemistry* Stereoisomerism Temperature |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Dipeptides; 0/Hydroxy Acids; 0/Ligands; 325-69-9/2-fluorophenylalanine; 63-91-2/Phenylalanine; 7631-86-9/Silicon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Enantiomeric separation of chiral dipeptides by CE-ESI-MS employing a partial filling technique with...
Next Document: Sphenoid septations and their relationship with internal carotid arteries: anatomical and radiologic...