Document Detail


Enantioselective two-dimensional high-performance liquid chromatographic determination of N-methyl-d-aspartic acid and its analogues in mammals and bivalves.
MedLine Citation:
PMID:  22999197     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A validated and fully automated chiral 2D-HPLC system was developed for the simultaneous determination of N-methyl-d-aspartic acid (NMDA) analogues by combining a long microbore-monolithic ODS column (0.53mm i.d.×1000mm) and narrowbore-enantioselective columns (1.5mm i.d.×150 or 250mm). The target analytes, enantiomers of N-methylaspartic acid (NMA) and N-methylglutamic acid (NMG), were precolumn-derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and detected by their fluorescence. The values of the lower limit of quantification for these enantiomers were 2.5fmol. In the tissues and plasma of rats, neither NMA nor NMG were detected. On the other hand, in the mantle and foot of Scapharca broughtonii, a large amount of NMDA was present (170.1 and 43.5nmol/g), and the enantiomers of NMG were also observed. Meretrix lusoria contained NMDA (29.3nmol/g) and NMLG (13.8nmol/g), and Ruditapes philippinarum contained only NMLG (2.6nmol/g). The obtained results were confirmed using three different enantioselective columns and also using a 2D-HPLC-MS/MS system. These results indicated that neuroactive d-amino acid, NMDA, and its analogues were present in animals, and their physiological significance is expected to be clarified.
Authors:
Reiko Koga; Yurika Miyoshi; Eiichi Negishi; Tsuneaki Kaneko; Masashi Mita; Wolfgang Lindner; Kenji Hamase
Related Documents :
22242677 - In situ study of the deposition of (ultra)thin organic phosphonic acid layers on the ox...
10746877 - Caffeic acid oligomers in lithospermum erythrorhizon cell suspension cultures.
22272107 - Compositional studies: antioxidant and antidiabetic activities of capparis decidua (for...
16824717 - Cytotoxic, antioxidant and antibacterial activities of varthemia iphionoides boiss. ext...
7525967 - Mutational analysis of the fingers and palm subdomains of human immunodeficiency virus ...
8612047 - Application of chemical cytochrome p-450 model systems to studies on drug metabolism--v...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-31
Journal Detail:
Title:  Journal of chromatography. A     Volume:  -     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-9-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Novel amide polar-embedded reversed-phase column for the fast liquid chromatography-tandem mass spec...
Next Document:  Pulsed electromembrane extraction: A new concept of electrically enhanced extraction.