Document Detail


Enantioselective synthesis of (S)-2-cyano-2-methylpentanoic acid by nitrilase.
MedLine Citation:
PMID:  23288294     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The nitrilase gene of Rhodococcus rhodochrous J1 was expressed in Escherichia coli using the expression vector, pKK223-3. The recombinant E. coli JM109 cells hydrolyzed enantioselectively 2-methyl-2-propylmalononitrile to form (S)-2-cyano-2-methylpentanoic acid (CMPA) with 96 % e.e. Under optimized conditions, 80 g (S)-CMPA l(-1) was produced with a molar yield of 97 % at 30 °C after a 24 h without any by-products.
Authors:
Toyokazu Yoshida; Koichi Mitsukura; Takuya Mizutani; Ryo Nakashima; Yasuyo Shimizu; Hiroshi Kawabata; Toru Nagasawa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-4
Journal Detail:
Title:  Biotechnology letters     Volume:  -     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Biomolecular Science, Gifu University, Yanagido 1-1, Gifu, 501-1193, Japan, toyosida@gifu-u.ac.jp.
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