Document Detail


Enantioselective epoxidation of linolenic acid catalysed by cytochrome P450(BM3) from Bacillus megaterium.
MedLine Citation:
PMID:  16032346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cytochrome P450(BM3), from Bacillus megaterium, catalyses the epoxidation of linolenic acid yielding 15,16-epoxyoctadeca-9,12-dienoic acid with complete regio- and moderate enantio-selectivity (60% ee). The absolute configuration of the product is tentatively assigned as 15(R),16(S)-. The Michaelis-Menten parameters kcat and Km for the reaction were determined to be 3126 +/- 226 min(-1) and 24 +/- 6 microM respectively.
Authors:
Ayhan Celik; Davide Sperandio; Robert E Speight; Nicholas J Turner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-06-16
Journal Detail:
Title:  Organic & biomolecular chemistry     Volume:  3     ISSN:  1477-0520     ISO Abbreviation:  Org. Biomol. Chem.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-20     Completed Date:  2006-06-28     Revised Date:  2007-08-13    
Medline Journal Info:
Nlm Unique ID:  101154995     Medline TA:  Org Biomol Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  2688-90     Citation Subset:  IM    
Affiliation:
School of Chemistry, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, UK EH9 3JJ.
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MeSH Terms
Descriptor/Qualifier:
Bacillus megaterium / enzymology*
Catalysis
Cytochrome P-450 Enzyme System / metabolism*
Epoxy Compounds / metabolism*
Stereoisomerism
alpha-Linolenic Acid / metabolism*
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Epoxy Compounds; 463-40-1/alpha-Linolenic Acid; 9035-51-2/Cytochrome P-450 Enzyme System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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