Document Detail


Enantioresolution of five β-blockers by reversed-phase high-performance liquid chromatography using fifteen chiral derivatizing reagents having amino acids or their amides as chiral auxiliaries on a cyanuric chloride platform.
MedLine Citation:
PMID:  21678456     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Enantioseparation of five β-blockers, namely, (R,S)-atenolol, (R,S)-propranolol, (R,S)-bisoprolol, (R,S)-metoprolol and (R,S)-carvedilol, was achieved as their diastereomers prepared with chiral derivatizing reagents (CDRs) synthesized on a cyanuric chloride platform. Fifteen CDRs were synthesized by nucleophilic substitution of the Cl atom in cyanuric chloride or its 6-methoxy derivative with amino acids (namely, l-Leu, l-Val, d-Phg, l-Met and l-Ala) or their amides as chiral auxiliaries. The diastereomers were synthesized under microwave irradiation for 70 or 100 s at 85% power. Separation of diastereomers was carried out on a C(18) column and gradient eluting mixtures of methanol with aqueous trifluoroacetic acid with UV detection at 230 nm. Separation efficiencies of the reagents were compared on the basis of effect of chiral auxiliaries (i.e. amino acids or amino acid amides) and achiral substituents (i.e. chlorine or methoxy group) in the CDRs. The method was validated for detection limit, linearity, accuracy and precision. Copyright © 2011 John Wiley & Sons, Ltd.
Authors:
Ravi Bhushan; Shuchi Dixit
Related Documents :
7092916 - A biochemical study of the cotton pellet granuloma in the rat. effects of dexamethasone...
94746 - Fungal nucleic acids as interferon inducers.
5449126 - Biosynthesis of caffeine in tea callus tissue.
1376126 - Effect of saffron on cell colony formation and cellular nucleic acid and protein synthe...
23313356 - Homocysteine-lowering therapy with folic acid is effective in cardiovascular disease pr...
23607666 - Characterization and quantification of mining-related 'naphthenic acids' in groundwater...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-16
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  -     ISSN:  1099-0801     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 John Wiley & Sons, Ltd.
Affiliation:
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India. rbushfcy@iitr.ernet.in.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The Efficacy and Safety of Sildenafil in Patients With Pulmonary Arterial Hypertension Associated Wi...
Next Document:  Simple HPLC method for detection of trace ephedrine and pseudoephedrine in high-purity methamphetami...