Document Detail

Enamel pits in hamster molars, formed by a single high fluoride dose, are associated with a perturbation of transitional stage ameloblasts.
MedLine Citation:
PMID:  22947666     Owner:  NLM     Status:  MEDLINE    
Excessive intake of fluoride (F) by young children results in the formation of enamel subsurface porosities and pits, called enamel fluorosis. In this study, we used a single high dose of F administered to hamster pups to determine the stage of ameloblasts most affected by F and whether pit formation was related to F-related sub-ameloblastic cyst formation. Hamster pups received a single subcutaneous injection of either 20 mg or 40 mg NaF/kg body weight, were sacrificed 24 h later, and the number of cysts formed in the first molars were counted. Other pups were sacrificed 8 days after F injection, when the first molars had just erupted, to score for enamel defects. All F-injected pups formed enamel defects in the upper half of the cusps in a dose-dependent way. After injection of 20 mg NaF/kg, an average of 2.5 white spots per molar was found but no pits. At 40 mg NaF/kg, almost 4.5 spots per molar were counted as well as 2 pits per molar. The defects in erupted enamel were located in the upper half of the cusps, sites where cysts had formed at the transition stage of ameloblast differentiation. These results suggest that transitional ameloblasts, located between secretory- and maturation-stage ameloblasts, are most sensitive to the effects of a single high dose of F. F-induced cysts formed earlier at the pre-secretory stage were not correlated to either white spots or enamel pits, suggesting that damaged ameloblasts overlying a F-induced cyst regenerate and continue to form enamel.
D M Lyaruu; L Vermeulen; N Stienen; T J M Bervoets; P K Denbesten; A L J J Bronckers
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-08-28
Journal Detail:
Title:  Caries research     Volume:  46     ISSN:  1421-976X     ISO Abbreviation:  Caries Res.     Publication Date:  2012  
Date Detail:
Created Date:  2012-12-13     Completed Date:  2013-05-20     Revised Date:  2013-08-30    
Medline Journal Info:
Nlm Unique ID:  0103374     Medline TA:  Caries Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  575-80     Citation Subset:  D; IM    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Department of Oral Cell Biology, ACTA, University of Amsterdam and VU University of Amsterdam, MOVE-Research Institute, Amsterdam, The Netherlands.
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MeSH Terms
Ameloblasts / drug effects*,  pathology
Cysts / chemically induced
Dental Enamel / drug effects*
Enamel Organ / drug effects,  physiology*
Fluorosis, Dental / pathology*
Plastic Embedding
Sodium Fluoride / administration & dosage,  adverse effects*
Grant Support
Reg. No./Substance:
7681-49-4/Sodium Fluoride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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