| Enamel Matrix Protein Adsorption to Root Surfaces in the Presence or Absence of Human Blood. | |
| | |
MedLine Citation:
|
PMID: 22122520 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Background: The clinical use of an enamel matrix derivative (EMD) has been shown to promote formation of new cementum, periodontal ligament and bone and to significantly enhance the clinical outcomes following regenerative periodontal surgery. At the time being it is unknown to what extent the bleeding during periodontal surgery may compete with EMD adsorption to root surfaces. Aim: To evaluate the effect of blood interactions on EMD adsorption to root surfaces mimicking various clinical settings and to test their ability to influence human periodontal ligament (PDL) cell attachment and proliferation. Material and Methods: Teeth extracted for orthodontic reasons were subjected to ex vivo scaling and root planing, treated with 24% ethylenediamintetraacetic acid (EDTA), EMD and/or human blood in 6 clinically related settings to determine the ability for EMD to adsorb to root surfaces. Surfaces were analyzed for protein adsorption via scanning electron microscopy and immunohistochemical staining with an anti-EMD antibody. Primary human PDL cells were seeded on root surfaces and quantified for cell attachment and cell proliferation. Results: Plasma proteins from blood samples altered the ability for EMD to adsorb to root surfaces on human teeth. Samples coated with EMD lacking blood demonstrated a consistent even layer of EMD adsorption to the root surface. In vitro experiments with PDL cells demonstrated improved cell attachment and proliferation in all samples coated with EMD (irrespective of EDTA) when compared to samples containing human blood. Conclusion: Based on these findings, it is advised to minimize blood interactions during periodontal surgeries in order to allow better adsorption of EMD to root surfaces. |
| | |
Authors:
|
Richard J Miron; Dieter D Bosshardt; Oliver Laugisch; Christos Katsaros; Daniel Buser; Anton Sculean |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-28 |
Journal Detail:
|
Title: Journal of periodontology Volume: - ISSN: 1943-3670 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8000345 Medline TA: J Periodontol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Deparment of Periodontology. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cigarette Smoke Extract Induces Select MMP and Integrin Expression in Periodontal Ligament Fibroblas...
Next Document: Interleukin-6 Gene Promoter Methylation in Rheumatoid Arthritis and Chronic Periodontitis.