Document Detail


Enabling screening in 3D microenvironments: probing matrix and stromal effects on the morphology and proliferation of T47D breast carcinoma cells.
MedLine Citation:
PMID:  23340769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During breast carcinoma progression, the three-dimensional (3D) microenvironment is continuously remodeled, and changes in the composition of the extracellular matrix (ECM) occur. High throughput screening platforms have been used to decipher the complexity of the microenvironment and to identify ECM components responsible for cancer progression. However, traditional screening platforms are typically limited to two-dimensional (2D) cultures, and often exclude the influence of ECM and stromal components. In this work, a system that integrates 3-dimensional cell culture techniques with an automated microfluidic platform was used to create a new ECM screening platform that cultures cells in more physiologically relevant 3D in vitro microenvironments containing stromal cells and different ECM molecules. This new ECM screening platform was used to culture T47D breast carcinoma cells in mono- and co-culture with human mammary fibroblasts (HMF) with seven combinations of three different ECM proteins (collagen, fibronectin, laminin). Differences in the morphology of T47D clusters, and the proliferation of T47D cells were found in ECM compositions rich in fibronectin or laminin. In addition, an MMP enzyme activity inhibition screening showed the capabilities of the platform for small molecule screening. The platform presented in this work enables screening for the effects of matrix and stromal compositions and show promises for providing new insights in the identification of key ECM components involved in breast cancer.
Authors:
Sara I Montanez-Sauri; Kyung Eun Sung; Erwin Berthier; David J Beebe
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  5     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-25     Completed Date:  2013-09-24     Revised Date:  2014-03-31    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  631-40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Automation
Breast / cytology
Breast Neoplasms / pathology*
Cell Culture Techniques*
Cell Line, Tumor
Cell Proliferation
Coculture Techniques
Disease Progression
Extracellular Matrix / metabolism*
Female
Fibronectins / metabolism
Humans
Image Processing, Computer-Assisted
Laminin / metabolism
Polymers / chemistry
Grant Support
ID/Acronym/Agency:
5T15LM007359/LM/NLM NIH HHS; P30 CA014520/CA/NCI NIH HHS; R01 EB010039/EB/NIBIB NIH HHS; R33 CA137673/CA/NCI NIH HHS; R33CA137673/CA/NCI NIH HHS; T15 LM007359/LM/NLM NIH HHS; T32 GM008349/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fibronectins; 0/Laminin; 0/Polymers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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