Document Detail


Employment of synchronized cells and flow microfluorometry in investigations on the JB-1 ascites tumour chalones.
MedLine Citation:
PMID:  127415     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In most experimental ascites tumours the growth rate decreases with increasing age and cell number. This decrease is caused by a prolongation of the cell cycle and an increasing accumulation of non-cycling cells in resting (or quiescent) G1 and G2 compartments. In cell-free ascitic fluid from the JB-1 ascites tumour in the plateau phase of growth lowmolecular-weight substances have been found which reversibly and specifically arrest JB-1 cells in G1 and G2. The present paper describes an in-vitro model for testing the effect of the humoral growth inhibitors contained in the ascitic fluid. The test system is based on synchronized JB-1 cells analysed by flow-through cytofluorometry. Addition to the synchronous cells of a ultrafiltrate (less than 50000 Daltons) of the JB-1 ascitic fluid was found to induce a complete, but temporary arrest of the cells at the G1-S border.
Authors:
P Bichel; N M Barfod; A Jakobsen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Virchows Archiv. B: Cell pathology     Volume:  19     ISSN:  0340-6075     ISO Abbreviation:  Virchows Arch B Cell Pathol     Publication Date:  1975 Nov 
Date Detail:
Created Date:  1976-01-23     Completed Date:  1976-01-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0437105     Medline TA:  Virchows Arch B Cell Pathol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  127-33     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascitic Fluid
Cell Division
Fluorometry
Growth Inhibitors / analysis*
Mice
Neoplasm Transplantation
Neoplasms, Experimental
Plasmacytoma / analysis*
Time Factors
Transplantation, Autologous
Chemical
Reg. No./Substance:
0/Growth Inhibitors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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