Document Detail

Emerging applications of sperm, embryo and somatic cell cryopreservation in maintenance, relocation and rederivation of swine genetics.
MedLine Citation:
PMID:  22898022     Owner:  NLM     Status:  MEDLINE    
Advances in porcine assisted reproductive technology (ART) make it possible to use cryopreserved sperm, embryos and somatic cells in the maintenance, relocation and regeneration of swine genetics. In this review, development of key application-limiting technology is discussed in each cell type, focusing on the efficiencies, ease of storage and transportation, and minimization of pathogen transmission. Methods to regenerate swine genetics and/or models using frozen sperm, embryos and somatic cells in combination with other porcine ARTs, such as in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), and somatic cell nuclear transplantation (SCNT), are also discussed. The applications of these ARTs utilizing cryopreserved cells will greatly increase the efficiency as well as biosecurity for maintenance, relocation and rederivation of swine genetics/models.
H Men; E M Walters; H Nagashima; R S Prather
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2012-08-13
Journal Detail:
Title:  Theriogenology     Volume:  78     ISSN:  1879-3231     ISO Abbreviation:  Theriogenology     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-15     Completed Date:  2013-04-05     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1720-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA.
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MeSH Terms
Cloning, Organism
Cryopreservation / methods,  veterinary*
Embryo, Mammalian*
Fertilization in Vitro / veterinary
Nuclear Transfer Techniques / veterinary
Reproductive Techniques, Assisted / veterinary*
Semen Preservation / methods,  veterinary*
Sperm Injections, Intracytoplasmic / veterinary
Sus scrofa / genetics
Swine / genetics*
Grant Support
R21 RR025879/RR/NCRR NIH HHS; R21-RR025879/RR/NCRR NIH HHS; U42 OD011140/OD/NIH HHS; U42 RR018877/RR/NCRR NIH HHS; U42-RR018877/OD011140/OD/NIH HHS

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