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Emerging Trend of Mutation Profile of rpoB Gene in MDR Tuberculosis, North India.
MedLine Citation:
PMID:  24082462     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The present study was conducted on North Indian population to observe rpoB gene mutation profile in multidrug resistant Mycobacterium tuberculosis. This was an observational study. 30 cases of MDR-TB proven by culture and drug sensitivity were selected. DNA sequencing of 81 bp (codon 507-533) long RRDR of Mycobacterium tuberculosis was done to detect the sites of mutation. Out of 30 cases, 24 showed a single mutation in the RRDR region of rpoB gene in which 16 (53.33 %) showed mutation in codon 531(TCG→TTG), 5 cases (16.66 %) showed mutation in codon 526(CAC→TAC), mutation in codon 516(GAC→GTC, AAC) was present in 3 cases (10 %). It was also observed that mutation in more than one codon was present in 4 cases (13.33 %), which included deletion at codon 509(AGC→-GC), mutation at 513(CAA→CTA), 516, 526, 529(CGA→CTA) and 531. No mutation was detected in RRDR in 2 cases (6.66 %). Our finding of 13.33 % cases with multiple sites of mutation in RRDR region is in contrast to earlier studies done in North India which showed single mutation detected in RRDR of rpoB gene that highlights the emerging change in the trend of mutation profile of rpoB gene in rifampicin resistant Mycobacterium tuberculosis.
Authors:
Jemil S Makadia; Anju Jain; Surajeet Kumar Patra; B L Sherwal; Ashwani Khanna
Publication Detail:
Type:  Journal Article     Date:  2012-06-07
Journal Detail:
Title:  Indian journal of clinical biochemistry : IJCB     Volume:  27     ISSN:  0970-1915     ISO Abbreviation:  Indian J Clin Biochem     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2013-10-01     Completed Date:  2013-10-01     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  8708303     Medline TA:  Indian J Clin Biochem     Country:  India    
Other Details:
Languages:  eng     Pagination:  370-4     Citation Subset:  -    
Affiliation:
Department of Biochemistry, Lady Hardinge Medical College, New Delhi, India.
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