Document Detail


Emerging roots alter epidermal cell fate through mechanical and reactive oxygen species signaling.
MedLine Citation:
PMID:  22904148     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A central question in biology is how spatial information is conveyed to locally establish a developmental program. Rice (Oryza sativa) can survive flash floods by the emergence of adventitious roots from the stem. Epidermal cells that overlie adventitious root primordia undergo cell death to facilitate root emergence. Root growth and epidermal cell death are both controlled by ethylene. This study aimed to identify the signal responsible for the spatial control of cell death. Epidermal cell death correlated with the proximity to root primordia in wild-type and ADVENTITIOUS ROOTLESS1 plants, indicating that the root emits a spatial signal. Ethylene-induced root growth generated a mechanical force of ~18 millinewtons within 1 h. Force application to epidermal cells above root primordia caused cell death in a dose-dependent manner and was inhibited by 1-methylcyclopropene or diphenylene iodonium, an inhibitor of NADPH oxidase. Exposure of epidermal cells not overlying a root to either force and ethylene or force and the catalase inhibitor aminotriazole induced ectopic cell death. Genetic downregulation of the reactive oxygen species (ROS) scavenger METALLOTHIONEIN2b likewise promoted force-induced ectopic cell death. Hence, reprogramming of epidermal cell fate by the volatile plant hormone ethylene requires two signals: mechanosensing for spatial resolution and ROS for cell death signaling.
Authors:
Bianka Steffens; Alexander Kovalev; Stanislav N Gorb; Margret Sauter
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Publication Detail:
Type:  Journal Article     Date:  2012-08-17
Journal Detail:
Title:  The Plant cell     Volume:  24     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-09-28     Completed Date:  2013-02-13     Revised Date:  2013-08-12    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3296-306     Citation Subset:  IM    
Affiliation:
Plant Developmental Biology and Plant Physiology, Institute of Botany, University of Kiel, 24118 Kiel, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amitrole / pharmacology
Biomechanics
Cell Death
Cyclopropanes / pharmacology
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Ethylenes / pharmacology
NADPH Oxidase / antagonists & inhibitors
Onium Compounds / pharmacology
Organophosphorus Compounds / pharmacology
Oryza sativa / drug effects,  metabolism,  physiology*
Plant Cells / metabolism,  physiology
Plant Epidermis / cytology,  drug effects,  metabolism,  physiology*
Plant Proteins / antagonists & inhibitors,  metabolism
Plant Roots / drug effects,  metabolism,  physiology*
Plant Stems / drug effects,  metabolism,  physiology
Reactive Oxygen Species / metabolism*
Signal Transduction*
Stress, Mechanical
Chemical
Reg. No./Substance:
0/Cyclopropanes; 0/Enzyme Inhibitors; 0/Ethylenes; 0/Onium Compounds; 0/Organophosphorus Compounds; 0/Plant Proteins; 0/Reactive Oxygen Species; 16672-87-0/ethephon; 244-54-2/diphenyleneiodonium; 3100-04-7/1-methylcyclopropene; 61-82-5/Amitrole; 91GW059KN7/ethylene; EC 1.6.3.1/NADPH Oxidase
Comments/Corrections

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