Document Detail

Emergency procedure for digoxin radioassay.
MedLine Citation:
PMID:  688618     Owner:  NLM     Status:  MEDLINE    
A rapid but precise radioassay for digoxin has been developed by optimizing the conditions for antigen-antibody interaction and for separation of bound and free fractions. This new procedure involves incubation of radioactively labeled antigen, antibody, and standard or sample at 3 degrees C for 10 min. Charcoal is then added, the mixture centrifuged for 5 min, and the radioactivity of the supernate counted. Results are available in less than 1 h after receipt of the specimens, making the procedure suitable for emergency determinations. Results correlate well with those by our routine procedure (Becton Dickinson) (r = 0.9739, y = 0.0356 + 0.9915x, n = 49). Analytical recovery of added pure digoxin exceeds 94%, both within and between assay CV's are less than 8%.
I W Chen; M Sperling; C Volle; H R Maxon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  24     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1978 Sep 
Date Detail:
Created Date:  1978-12-02     Completed Date:  1978-12-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1564-7     Citation Subset:  IM    
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MeSH Terms
Antigen-Antibody Reactions
Digoxin / blood*
Radioimmunoassay / methods
Reg. No./Substance:
16291-96-6/Charcoal; 20830-75-5/Digoxin

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