Document Detail


Emergence of Klebsiella pneumoniae isolates producing inducible DHA-1 beta-lactamase in a university hospital in Taiwan.
MedLine Citation:
PMID:  12202541     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ten nonrepetitive clinical isolates of Klebsiella pneumoniae exhibiting an unusual inducible beta-lactam resistance phenotype were identified between January 1999 and September 2001 in a university hospital in Taiwan. In the presence of 2 micro g of clavulanic acid, the isolates showed a one to four twofold concentration increase in the MICs of ceftazidime, cefotaxime, and aztreonam but remained susceptible to cefepime (MICs, </=0.5 micro g/ml) and imipenem (MICs, </=0.5 micro g/ml). PCR, sequence analysis, and isoelectric focusing revealed production by these isolates of TEM-1, SHV-11, and DHA-1, a plasmid-encoded inducible AmpC beta-lactamase originally found in a Salmonella enterica serovar Enteritidis strain. Transfer of the resistance by conjugation experiments was not successful, but Southern hybridization showed that bla(DHA-1) was located on 70-kb plasmids, suggesting that the bla(DHA-1)-containing plasmids in the K. pneumoniae isolates were non-self-transmissible. Five isolates were recovered from patients in two surgery wards and two intensive care units. Acquisition of the DHA-1 producers could be traced back to previous hospitalizations 1 to 5 months earlier for the other five patients. Six and seven patterns among the isolates were demonstrated by plasmid analysis and ribotyping, respectively, indicating that the spread of the DHA-1 producers was due to both horizontal transfer of bla(DHA-1) and dissemination of endemic clones.
Authors:
Jing-Jou Yan; Wen-Chien Ko; Yun-Chih Jung; Chin-Luan Chuang; Jiunn-Jong Wu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  40     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-08-30     Completed Date:  2002-11-14     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3121-6     Citation Subset:  IM    
Affiliation:
Department of Pathology, College of Medicine, National Cheng Kung University. Department of Pathology, Sinlau Christian Hospital, Tainan, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Anti-Bacterial Agents / pharmacology*
Bacterial Proteins*
Conjugation, Genetic
Drug Resistance, Bacterial / genetics
Enzyme Induction
Female
Hospitals, University*
Humans
Klebsiella Infections / epidemiology*,  microbiology*
Klebsiella pneumoniae / drug effects,  enzymology*,  isolation & purification
Male
Microbial Sensitivity Tests
Middle Aged
Plasmids / genetics
Ribotyping
Taiwan / epidemiology
beta-Lactamases / metabolism*
beta-Lactams
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/beta-Lactams; EC 3.5.2.6/AmpC beta-lactamases; EC 3.5.2.6/beta-Lactamases
Comments/Corrections

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