| Embryotropic role of hemoglobin and ethylenediaminetetraacetic acid in preimplantation development of ICR mouse 1-cell embryos. | |
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MedLine Citation:
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PMID: 11056248 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To evaluate embryotropic action of hemoglobin (Hb) and ethylenediaminetetraacetic acid (EDTA) on preimplantation embryo development. DESIGN: In vitro model study using mouse embryos. SETTING: University affiliated hospital, Pochon CHA University. ANIMALS: Four-week-old block strain ICR mice naturally mated after superovulation. INTERVENTION(S): One-cell embryos were cultured in serum-free, modified preimplantation-1 medium, to which 1 microg/ml Hb and/or 0.1 mM EDTA were added. MAIN OUTCOME MEASURE(S): Preimplantation development and blastomere number. RESULT(S): More (P<.05) 1-cell embryos developed to the 4-cell (52% vs. 67%-84%), 8-cell (48% vs. 65%-81%), and blastocyst (40% vs. 61%-79%) stages after the addition of hemoglobin (Hb) and/or EDTA than after no addition. Highest proportion of embryos developed to each stage after the combined addition of Hb+EDTA. EDTA specifically stimulated the development before the 8-cell stage, which was as similar as Hb+EDTA. On the contrary, higher ratio of morula to blastocyst transformation was obtained after the addition of Hb or Hb+EDTA than after no addition (0.76 vs. 0.96-0.98). Significant increases in the cell number of blastocysts (46.5-47.2 vs. 53.2 cells), inner cell mass (ICM) cells (16.7-17.5 vs. 21 cells), and the ratio of ICM cells to trophoblasts (0.3-0.37 to 0.39) were found after the combined addition of Hb+EDTA, compared with no addition or with the addition of EDTA or Hb alone. CONCLUSIONS: Hb and EDTA have stage-specific effects on supporting preimplantation embryo development; Hb promotes both the development before the 8-cell stage and the morula to blastocyst transformation, whereas EDTA mainly promotes the development to the 8-cell stage. The combined exposure of embryos to Hb and EDTA improves not only preimplantation development but also the growth and quality of blastocysts. |
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Authors:
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S E Park; H M Chung; J J Ko; B C Lee; K Y Cha; J M Lim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Fertility and sterility Volume: 74 ISSN: 0015-0282 ISO Abbreviation: Fertil. Steril. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-11-27 Completed Date: 2000-12-07 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 996-1000 Citation Subset: IM |
Affiliation:
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Infertility Medical Center of CHA General Hospital, College of Medicine, Pochon CHA University, Seoul, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / drug effects, physiology Chelating Agents / pharmacology* Culture Techniques Drug Combinations Edetic Acid / pharmacology* Embryo, Mammalian / drug effects*, physiology Embryonic Development* / drug effects Embryonic and Fetal Development / drug effects Female Hemoglobins / pharmacology* Mice Mice, Inbred ICR Morula / physiology Pregnancy |
| Chemical | |
Reg. No./Substance:
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0/Chelating Agents; 0/Drug Combinations; 0/Hemoglobins; 60-00-4/Edetic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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