Document Detail


Embryotoxicity of micropore filters used in liquid sterilization.
MedLine Citation:
PMID:  2077089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryo-toxicity in the first 5 ml of filtrate and the three larger-capacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized.
Authors:
K L Harrison; D A Sherrin; T A Hawthorne; T M Breen; G A West; L M Wilson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of in vitro fertilization and embryo transfer : IVF     Volume:  7     ISSN:  0740-7769     ISO Abbreviation:  J In Vitro Fert Embryo Transf     Publication Date:  1990 Dec 
Date Detail:
Created Date:  1991-04-24     Completed Date:  1991-04-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8412594     Medline TA:  J In Vitro Fert Embryo Transf     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  347-50     Citation Subset:  IM    
Affiliation:
Queensland Fertility Group, St. Andrews Hospital, Brisbane, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Embryo, Mammalian / drug effects,  pathology*
Embryonic and Fetal Development / drug effects,  physiology
Ethylene Oxide / diagnostic use,  toxicity
Mice
Micropore Filters*
Sterilization / instrumentation*,  methods
Chemical
Reg. No./Substance:
75-21-8/Ethylene Oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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