| Embryotoxicity of micropore filters used in liquid sterilization. | |
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MedLine Citation:
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PMID: 2077089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryo-toxicity in the first 5 ml of filtrate and the three larger-capacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized. |
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Authors:
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K L Harrison; D A Sherrin; T A Hawthorne; T M Breen; G A West; L M Wilson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of in vitro fertilization and embryo transfer : IVF Volume: 7 ISSN: 0740-7769 ISO Abbreviation: J In Vitro Fert Embryo Transf Publication Date: 1990 Dec |
Date Detail:
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Created Date: 1991-04-24 Completed Date: 1991-04-24 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8412594 Medline TA: J In Vitro Fert Embryo Transf Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 347-50 Citation Subset: IM |
Affiliation:
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Queensland Fertility Group, St. Andrews Hospital, Brisbane, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Embryo, Mammalian / drug effects, pathology* Embryonic and Fetal Development / drug effects, physiology Ethylene Oxide / diagnostic use, toxicity Mice Micropore Filters* Sterilization / instrumentation*, methods |
| Chemical | |
Reg. No./Substance:
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75-21-8/Ethylene Oxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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