Document Detail

Embryonic stem cells produce neurotrophins in response to cerebral tissue extract: Cell line-dependent differences.
MedLine Citation:
PMID:  17335079     Owner:  NLM     Status:  MEDLINE    
In the present study, we compare the capacity of two different embryonic stem (ES) cell lines to secrete neurotrophins in response to cerebral tissue extract derived from healthy or injured rat brains. The intrinsic capacity of the embryonic cell lines BAC7 (feeder cell-dependent cultivation) to release brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3) exceeded the release of these factors by CGR8 cells (feeder cell-free growth) by factors of 10 and 4, respectively. Nerve growth factor (NGF) was secreted only by BAC7 cells. Conditioning of cell lines with cerebral tissue extract derived from healthy or fluid percussion-injured rat brains resulted in a significant time-dependent increase in BDNF release in both cell lines. The increase in BDNF release by BAC7 cells was more pronounced when cells were incubated with brain extract derived from injured brain. However, differences in neurotrophin release associated with the origin of brain extract were at no time statistically significant. Neutrophin-3 and NGF release was inhibited when cell lines were exposed to cerebral tissue extract. The magnitude of the response to cerebral tissue extract was dependent on the intrinsic capacity of the cell lines to release neurotrophins. Our results clearly demonstrate significant variations in the intrinsic capability of different stem cell lines to produce neurotrophic factors. Furthermore, a significant modulation of neurotrophic factor release was observed following conditioning of cell lines with tissue extract derived from rat brains. A significant modulation of neurotrophin release dependent on the source of cerebral tissue extract used was not observed.
Kristine Bentz; Marek Molcanyi; Peter Riess; Andrea Elbers; Esther Pohl; Agapios Sachinidis; Jürgen Hescheler; Edmund Neugebauer; Ute Schäfer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  85     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-27     Completed Date:  2007-07-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1057-64     Citation Subset:  IM    
Copyright Information:
(c) 2007 Wiley-Liss, Inc.
Institute of Developmental Genetics, GSF-National Research Centre for Environment and Health, Munich, Germany.
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MeSH Terms
Brain Chemistry
Brain Tissue Transplantation / methods
Brain-Derived Neurotrophic Factor / metabolism,  secretion
Cell Differentiation / drug effects,  physiology
Cell Extracts / pharmacology
Cell Line
Cerebral Cortex / cytology,  metabolism*
Culture Media, Conditioned / pharmacology
Embryonic Stem Cells / metabolism*,  secretion*
Nerve Growth Factors / metabolism*,  secretion*
Nerve Regeneration / drug effects,  physiology
Neurotrophin 3 / metabolism,  secretion
Stem Cell Transplantation / methods
Time Factors
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 0/Cell Extracts; 0/Culture Media, Conditioned; 0/Nerve Growth Factors; 0/Neurotrophin 3

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