| Embryonic stem cell virus, a recombinant murine retrovirus with expression in embryonic stem cells. | |
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MedLine Citation:
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PMID: 2251265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The expression of Moloney murine leukemia virus and vectors derived from it is restricted in undifferentiated mouse embryonal carcinoma and embryonal stem (ES) cells. We have developed a retroviral vector, the murine embryonic stem cell virus (MESV), that is active in embryonal carcinoma and ES cells. MESV was derived from a retroviral mutant [PCC4-cell-passaged myeloproliferative sarcoma virus (PCMV)] expressed in embryonal carcinoma cells but not in ES cells. The enhancer region of PCMV was shown to be functional in both cell types, but sequences within the 5' untranslated region of PCMV were found to restrict viral expression in ES cells. Replacement of this region by related sequences obtained from the dl-587rev retrovirus results in MESV, a modified PCMV virus that confers G418 resistance to fibroblasts and ES cells with similar efficiencies. Expression of MESV in ES cells is mediated by transcriptional regulatory elements within the 5' long terminal repeat of the viral genome. |
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Authors:
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M Grez; E Akgün; F Hilberg; W Ostertag |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 87 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1990 Dec |
Date Detail:
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Created Date: 1991-01-16 Completed Date: 1991-01-16 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9202-6 Citation Subset: IM |
Affiliation:
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Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Abteilung Zellbiologie, Hamburg, Federal Republic of Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell Line DNA, Viral / genetics Embryo, Mammalian Enhancer Elements, Genetic Genetic Vectors Mice Molecular Sequence Data Moloney murine leukemia virus / genetics* Oligonucleotide Probes RNA, Viral / genetics Recombination, Genetic* Restriction Mapping Retroviridae / genetics* Teratoma |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/Oligonucleotide Probes; 0/RNA, Viral |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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