| Embryonic stem cell-derived cardiomyocytes as a model system to study cardioprotective effects of dexamethasone in doxorubicin cardiotoxicity. | |
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MedLine Citation:
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PMID: 19596060 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Embryonic stem cell (ESC)-derived beating cardiomyocytes may be considered as a suitable model for in vitro assessment of pharmacological and toxicological studies. In this model, laboratory animals are not required. In addition, physiological functions, such as heart beat, are assessed rather than single parameters such as cell viability. Here we report that doxorubicin (DOX) cardiotoxicity on mouse ESC-derived beating cardiomyocytes can be ameliorated by treatment with dexamethasone (DEX) when DEX is administrated only before DOX and not in combination with DOX. DEX effect appears to be mediated via glucocorticoid receptor and increases cardiomyocyte-specific gene expression. Cardiotoxicity of DOX can be augmented by calcium channel blocker, verapamil (VER) which also decreases the expression of cardiac gene markers. This model provides us with a clinical suggestion which proposes that the beneficial effect of DEX is obtained when DEX was added before DOX administration. |
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Authors:
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Mahboubeh Farokhpour; Khadijeh Karbalaie; Somayeh Tanhaei; Marzeyeh Nematollahi; Mahmoud Etebari; Hamid Mirmohammad Sadeghi; Mohammad Hossein Nasr-Esfahani; Hossein Baharvand |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-22 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 23 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-02 Completed Date: 2009-12-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 1422-8 Citation Subset: IM |
Affiliation:
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Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Esfahan, Iran. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibiotics, Antineoplastic / toxicity* Cytotoxins / toxicity Dexamethasone / pharmacology* Doxorubicin / toxicity* Embryonic Stem Cells / cytology* Gene Expression Regulation / drug effects Glucocorticoids / pharmacology* Heart / drug effects Mice Models, Biological* Myocardium / metabolism Myocytes, Cardiac / drug effects*, metabolism Protective Agents / pharmacology* Reverse Transcriptase Polymerase Chain Reaction Verapamil / toxicity |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Cytotoxins; 0/Glucocorticoids; 0/Protective Agents; 23214-92-8/Doxorubicin; 50-02-2/Dexamethasone; 52-53-9/Verapamil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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