Document Detail


Embryonic mouse spinal cord motor neuron hybrid cells.
MedLine Citation:
PMID:  1751804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies of motor neurons are difficult because of limitations in their isolation and culture. One solution is to produce clonal neural hybrid cells that can express motor neuron characteristics; we fused an aminopterin-sensitive and neomycin-resistant mouse neuroblastoma cell line to isolated embryonic mouse spinal cord motor neurons. Several hybrid neuron cell lines expressing high levels of choline acetyltransferase (CHAT) enzyme activity were found. These were cloned and clones with high CHAT activity isolated. The hybrid nature of cloned cells was confirmed by karyotyping and determining glucose phosphate isomerase allozymes. The availability of these embryonic clonal hybrid cells will enable molecular, physiological, and biochemical studies to define motor neuron-specific properties.
Authors:
E F Salazar-Grueso; S Kim; H Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroreport     Volume:  2     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1992-01-27     Completed Date:  1992-01-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  505-8     Citation Subset:  IM    
Affiliation:
Department of Neurology, University of Chicago, Il 60637.
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MeSH Terms
Descriptor/Qualifier:
Aminopterin / pharmacology
Animals
Cell Fusion
Choline O-Acetyltransferase / genetics
Chromosomes
Clone Cells
Drug Resistance
Gentamicins / pharmacology
Glucose-6-Phosphate Isomerase / analysis
Hybrid Cells / cytology*,  drug effects,  enzymology
Hypoxanthine Phosphoribosyltransferase / genetics
Isoenzymes / analysis
Mice
Mice, Inbred C57BL / embryology
Motor Neurons / cytology*
Neuroblastoma / pathology
Selection, Genetic
Spinal Cord / cytology,  embryology*
Tumor Cells, Cultured / pathology
Chemical
Reg. No./Substance:
0/Gentamicins; 0/Isoenzymes; 49863-47-0/antibiotic G 418; 54-62-6/Aminopterin; EC 2.3.1.6/Choline O-Acetyltransferase; EC 2.4.2.8/Hypoxanthine Phosphoribosyltransferase; EC 5.3.1.9/Glucose-6-Phosphate Isomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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