| Embryonic mouse spinal cord motor neuron hybrid cells. | |
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MedLine Citation:
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PMID: 1751804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies of motor neurons are difficult because of limitations in their isolation and culture. One solution is to produce clonal neural hybrid cells that can express motor neuron characteristics; we fused an aminopterin-sensitive and neomycin-resistant mouse neuroblastoma cell line to isolated embryonic mouse spinal cord motor neurons. Several hybrid neuron cell lines expressing high levels of choline acetyltransferase (CHAT) enzyme activity were found. These were cloned and clones with high CHAT activity isolated. The hybrid nature of cloned cells was confirmed by karyotyping and determining glucose phosphate isomerase allozymes. The availability of these embryonic clonal hybrid cells will enable molecular, physiological, and biochemical studies to define motor neuron-specific properties. |
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Authors:
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E F Salazar-Grueso; S Kim; H Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuroreport Volume: 2 ISSN: 0959-4965 ISO Abbreviation: Neuroreport Publication Date: 1991 Sep |
Date Detail:
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Created Date: 1992-01-27 Completed Date: 1992-01-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9100935 Medline TA: Neuroreport Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 505-8 Citation Subset: IM |
Affiliation:
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Department of Neurology, University of Chicago, Il 60637. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aminopterin
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pharmacology Animals Cell Fusion Choline O-Acetyltransferase / genetics Chromosomes Clone Cells Drug Resistance Gentamicins / pharmacology Glucose-6-Phosphate Isomerase / analysis Hybrid Cells / cytology*, drug effects, enzymology Hypoxanthine Phosphoribosyltransferase / genetics Isoenzymes / analysis Mice Mice, Inbred C57BL / embryology Motor Neurons / cytology* Neuroblastoma / pathology Selection, Genetic Spinal Cord / cytology, embryology* Tumor Cells, Cultured / pathology |
| Chemical | |
Reg. No./Substance:
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0/Gentamicins; 0/Isoenzymes; 49863-47-0/antibiotic G 418; 54-62-6/Aminopterin; EC 2.3.1.6/Choline O-Acetyltransferase; EC 2.4.2.8/Hypoxanthine Phosphoribosyltransferase; EC 5.3.1.9/Glucose-6-Phosphate Isomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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