| Embryonic mouse blood flow and oxygen correlate with early pancreatic differentiation. | |
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MedLine Citation:
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PMID: 21050843 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mammalian embryo represents a fundamental paradox in biology. Its location within the uterus, especially early during development when embryonic cardiovascular development and placental blood flow are not well-established, leads to an obligate hypoxic environment. Despite this hypoxia, the embryonic cells are able to undergo remarkable growth, morphogenesis, and differentiation. Recent evidence suggests that embryonic organ differentiation, including pancreatic β-cells, is tightly regulated by oxygen levels. Since a major determinant of oxygen tension in mammalian embryos after implantation is embryonic blood flow, here we used a novel survivable in utero intracardiac injection technique to deliver a vascular tracer to living mouse embryos. Once injected, the embryonic heart could be visualized to continue contracting normally, thereby distributing the tracer specifically only to those regions where embryonic blood was flowing. We found that the embryonic pancreas early in development shows a remarkable paucity of blood flow and that the presence of blood flow correlates with the differentiation state of the developing pancreatic epithelial cells in the region of the blood flow. |
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Authors:
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Sohail R Shah; Farzad Esni; Adam Jakub; Jose Paredes; Nikesh Lath; Marcus Malek; Douglas A Potoka; Krishna Prasadan; Pier G Mastroberardino; Chiyo Shiota; Ping Guo; Kelly A Miller; David J Hackam; R Cartland Burns; Sidhartha S Tulachan; George K Gittes |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-11-02 |
Journal Detail:
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Title: Developmental biology Volume: 349 ISSN: 1095-564X ISO Abbreviation: Dev. Biol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-10 Completed Date: 2011-02-24 Revised Date: 2012-01-17 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 342-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Division of Pediatric General and Thoracic Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh Medical Center, 45th Street and Penn Avenue, Pittsburgh, PA 15201, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cardiac Imaging Techniques / methods Cell Differentiation / physiology* Embryo, Mammalian / blood supply* Fluoresceins / administration & dosage Immunohistochemistry Mice Microscopy, Fluorescence Oxygen / metabolism* Pancreas / blood supply, cytology, embryology*, metabolism Plant Lectins / administration & dosage Ultrasonography, Interventional / methods* |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK064952/DK/NIDDK NIH HHS; R01 DK064952-05/DK/NIDDK NIH HHS; R01 DK064952-06/DK/NIDDK NIH HHS; R01 DK083541-01/DK/NIDDK NIH HHS; R01 DK083541-01/DK/NIDDK NIH HHS; R01 DK083541-02/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Plant Lectins; 0/tomato lectin; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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