Document Detail


Embryonic fibroblasts from mice lacking Tgif were defective in cell cycling.
MedLine Citation:
PMID:  16705179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Holoprosencephaly (HPE) is the most common structural anomaly of the human brain, resulting from incomplete cleavage of the developing forebrain during embryogenesis. Haploinsufficient mutations in the TG-interacting factor (TGIF) gene were previously identified in a subset of HPE families and sporadic patients, and this gene is located within a region of chromosome 18 that is associated with nonrandom chromosomal aberrations in HPE patients. TGIF is a three-amino-acid loop extension (TALE) homeodomain-containing transcription factor that functions both as a corepressor of the transforming growth factor beta (TGF-beta) pathway and as a competitor of the retinoic acid pathway. Here we describe mice deficient in Tgif that exhibited laterality defects and growth retardation and developed kinked tails. Cellular analysis of mutant mouse embryonic fibroblasts (MEFs) demonstrated for the first time that Tgif regulates proliferation and progression through the G1 cell cycle phase. Additionally, wild-type human TGIF was able to rescue this proliferative defect in MEFs. In contrast, a subset of human Tgif mutations detected in HPE patients was unable to rescue the proliferative defect. However, an absence of Tgif did not alter the normal inhibition of proliferation caused by treatment with TGF-beta or retinoic acid. Developmental control of proliferation by Tgif may play a role in the pathogenesis of HPE.
Authors:
Lynn Mar; Pamela A Hoodless
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  26     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-17     Completed Date:  2006-06-23     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4302-10     Citation Subset:  IM    
Affiliation:
Terry Fox Laboratory, British Columbia Cancer Research Centre, 675 West 10th Avenue, Vancouver, BC, Canada, V5Z 1L3.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle*
Cell Proliferation
Embryo, Mammalian / cytology*,  pathology
Embryonic Development
Fibroblasts / cytology*,  drug effects,  metabolism*,  pathology
Functional Laterality
Gene Expression Regulation, Developmental
Gene Targeting
Glucose Transport Proteins, Facilitative / deficiency*,  metabolism*
Homeodomain Proteins / metabolism
Mice
Phenotype
Transforming Growth Factor beta / pharmacology
Tretinoin / pharmacology
Chemical
Reg. No./Substance:
0/Glucose Transport Proteins, Facilitative; 0/Homeodomain Proteins; 0/Slc2a8 protein, mouse; 0/Transforming Growth Factor beta; 302-79-4/Tretinoin
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