Document Detail

Embryonic development of kisspeptin neurones in rat.
MedLine Citation:
PMID:  22530935     Owner:  NLM     Status:  Publisher    
Kisspeptins, encoded by the Kiss1 gene, play a key role in the regulation of reproductive function but nowadays very little is known about the ontogenesis of this system. The aims of the present study were to determine the period of arcuate nucleus (ARC) kisspeptin cell birth and the embryonic stage and neuroanatomical sites of onset of kisspeptin immunoreactivity. Bromo-desoxy-uridine (BrdU) was administered to female rats at various gestational stages and double immunohistochemistry against kisspeptin and BrdU was performed on brain sections from their offsprings. The period of neurogenesis of ARC kisspeptin neurones begun between embryonic day (E) E12.5 and E13.5, reached its peak at E15.5 and was not completely over at E17.5. Kiss1 mRNA was detected in mediobasal hypothalamic punches of embryos aged E14.5, E16.5, E18.5 and E22.5 by real-time RT-PCR. Accordingly, kisspeptin-immunoreactive (ir) cells were consistently detected in the embryonic ARC from E14.5 and their number increased until E18.5 to reach about half the level observed in adults. Between E18.5 and E22.5 the number of kisspeptin-ir cells and hypothalamic Kiss1 expression significantly decreased regardless of sex and this decrease persisted until birth. Taken together, these results demonstrate that rat ARC kisspeptin neurones are born locally during an extended embryonic period and are able to synthesise kisspeptin rapidly after their birth, consistent with the hypothesis of a role during embryonic activation of the hypothalamo-hypophysea-gonadal axis. A sex-independent decrease of kisspeptin-ir was observed during the perinatal period suggestive of important regulations of kisspeptin neurones around birth. © 2012 The Authors. Journal of Neuroendocrinology © 2012 Blackwell Publishing Ltd.
E Desroziers; M Droguerre; A H Bentsen; V Robert; J D Mikkelsen; A Caraty; Y Tillet; A Duittoz; I Franceschini
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-25
Journal Detail:
Title:  Journal of neuroendocrinology     Volume:  -     ISSN:  1365-2826     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8913461     Medline TA:  J Neuroendocrinol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors. Journal of Neuroendocrinology © 2012 Blackwell Publishing Ltd.
INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France CNRS, UMR7247, F-37380 Nouzilly, France. Université François Rabelais de Tours, F-37000 Tours, France IFCE, F-37380 Nouzilly, France. Neurobiology Research Unit, University Hospital Rigshospitalet, Copenhagen, Denmark.
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