Document Detail

Embryonic development in rabbits after insemination with spermatozoa stored at 37, 5 or-196 degrees C for various periods.
MedLine Citation:
PMID:  966235     Owner:  NLM     Status:  MEDLINE    
Rabbit spermatozoa stored at 37 degrees C for 5 hr, 5 degrees C for 5 hr or 5 days, and-196 degrees C for 5 hr, 5 days, or 1 year were used to artificially inseminate does induced to ovulate normal or excess numbers of eggs. Fertility and subsequent embryonic development were examined. Frozen spermatozoa stored for 5 hr fertilized fewer oocytes than those stored for 5 days or longer. Fewer embryos were recovered on Day 6 than on Day 2. Blastocysts were smaller when spermatozoa were stored for 5 days, were frozen or were placed in superovulating does, but no statistically significant differences in the % of fetal survival were found in the groups of normally ovulating does. Embryonic wastage associated with storage of spermatozoa resulted from a reduced fertilization rate and/or increased embryonic mortality before implantation.
R R Maurer; G F Stranzinger; S K Paufler
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  48     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1976 Sep 
Date Detail:
Created Date:  1976-12-01     Completed Date:  1976-12-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  43-9     Citation Subset:  IM    
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MeSH Terms
Blastocyst / physiology
Embryo, Mammalian / physiology*
Insemination, Artificial
Sperm Motility
Spermatozoa / physiology*
Time Factors
Tissue Preservation*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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