Document Detail

Embryonic abnormalities from misexpression of cNSCL1.
MedLine Citation:
PMID:  11573957     Owner:  NLM     Status:  MEDLINE    
cNSCL1 encodes a bHLH transcription factor and is specifically expressed in the developing nervous system. We used a replication-competent retrovirus to drive misexpression of cNSCL1 in chick embryos. We found that cNSCL1 misexpression was embryonic lethal and the embryos exhibited gross abnormalities. Many skeletal bones were abnormal and some were completely absent. Expression of BMP4 was reduced. The abnormalities were due to cNSCL1 misexpression in the systemic region, since microinjection of cNSCL1 retrovirus at one hindlimb primordium severely retarded its development, while other limbs on the same animal appeared normal. Similar misexpression of cNSCL2, a closely related bHLH gene, did not produce these phenotypes. Thus, the detrimental effects on embryonic development were specific to cNSCL1. These data indicate that cNSCL1 expression must be tightly regulated during development.
R T Yan; S Z Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  287     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-09-27     Completed Date:  2001-12-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  949-55     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Ophthalmology, University of Alabama at Birmingham School of Medicine, 700 South 18th Street, Birmingham, AL 35233, USA.
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MeSH Terms
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins / genetics,  metabolism
Bone and Bones / abnormalities,  embryology
Chick Embryo / abnormalities*,  growth & development
DNA-Binding Proteins / genetics,  metabolism*
Helix-Loop-Helix Motifs / genetics
Hindlimb / abnormalities,  embryology
In Situ Hybridization
Retroviridae / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/Bone Morphogenetic Protein 4; 0/Bone Morphogenetic Proteins; 0/DNA-Binding Proteins

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