Document Detail

Embryonic stem cells or induced pluripotent stem cells? A DNA integrity perspective.
MedLine Citation:
PMID:  23317057     Owner:  NLM     Status:  MEDLINE    
Induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) are two types of pluripotent stem cells that hold great promise for biomedical research and medical applications. iPSCs were initially favorably compared to ESCs. This view was first based on ethical arguments (the generation of iPSCs does not require the destruction of an embryo) and on immunological reasons (it is easier to derive patient HLA-matched iPSCs than ESCs). However, several reports suggest that iPSCs might be characterized by higher occurrence of epigenetic and genetic aberrations than ESCs as a consequence of the reprogramming process. We focus here on the DNA integrity of pluripotent stem cells and examine the three main sources of genomic abnormalities in iPSCs: (1) genomic variety of the parental cells, (2) cell reprogramming, and (3) in vitro cell culture. Recent reports claim that it is possible to generate mouse or human iPSC lines with a mutation level similar to that of the parental cells, suggesting that "genome-friendly" reprogramming techniques can be developed. The issue of iPSC DNA integrity clearly highlights the crucial need of guidelines to define the acceptable level of genomic integrity of pluripotent stem cells for biomedical applications. We discuss here the main issues that such guidelines should address.
Qiang Bai; Romain Desprat; Bernard Klein; Jean-Marc Lemaître; John De Vos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Current gene therapy     Volume:  13     ISSN:  1875-5631     ISO Abbreviation:  Curr Gene Ther     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-27     Completed Date:  2013-09-23     Revised Date:  2014-03-13    
Medline Journal Info:
Nlm Unique ID:  101125446     Medline TA:  Curr Gene Ther     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  93-8     Citation Subset:  IM    
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MeSH Terms
Cell Culture Techniques
Cell Differentiation
DNA / genetics*
Embryonic Stem Cells / cytology*,  immunology
Epigenesis, Genetic
Genomic Instability*
Induced Pluripotent Stem Cells / cytology*,  immunology
Reg. No./Substance:

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