Document Detail

Embryonic stem cells do not stiffen on rigid substrates.
MedLine Citation:
PMID:  20643049     Owner:  NLM     Status:  MEDLINE    
It has been previously established that living cells, including mesenchymal stem cells, stiffen in response to elevation of substrate stiffness. This stiffening is largely attributed to the elevation of the tractions at the cell base that is associated with increases in cell spreading on more-rigid substrates. We show here, surprisingly, that mouse embryonic stem cells (ESCs) do not stiffen when substrate stiffness increases. As shown recently, these cells do not increase spreading on more-rigid substrates either. However, these ESCs do increase their basal tractions as substrate stiffness increases. We conclude that these ESCs exhibit mechanical behaviors distinct from those of mesenchymal stem cells and of terminally differentiated cells, and decouple its apical cell stiffness from its basal tractional stresses during the substrate rigidity response.
Yeh-Chuin Poh; Farhan Chowdhury; Tetsuya S Tanaka; Ning Wang
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Publication Detail:
Type:  Letter; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Biophysical journal     Volume:  99     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-20     Completed Date:  2010-10-27     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L19-21     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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MeSH Terms
Biomechanical Phenomena / physiology
Cell Line
Embryonic Stem Cells / cytology,  physiology*
Stress, Mechanical
Grant Support
Erratum In:
Biophys J. 2011 Jan 5;100(1):268

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