Document Detail

Embryonal carcinoma and the basement membrane glycoproteins laminin and entactin.
MedLine Citation:
PMID:  8507559     Owner:  NLM     Status:  MEDLINE    
The mouse embryonal carcinoma lines PCC4-F and F9 have played important roles in the isolation and characterization of the two ubiquitous basement membrane proteins, laminin and entactin. The contributions of these cells to our work on extracellular matrices are briefly summarized. The in vitro differentiation of PCC4-F gives rise to a multiplicity of cell types. Two of these cell types have been propagated as cell lines. One of these, M1536-B3, synthesizes and deposits copious quantities of extracellular matrix glycoproteins, which led to the initial discovery and characterization of laminin and entactin. In addition, M1536-B3 provides a model system for analyzing the assembly of laminin and the laminin-entactin complex and for manipulating extracellular matrix structure and composition. The other cell line, 4CQ, synthesizes a matrix consisting of fibronectin and entactin. F9 cells differentiate to endodermal cells in response to retinoic acid and dibutyryl cyclic AMP (Strickland and Mahdavi, Cell 15: 393-402, 1978). The differentiated cells synthesize basement membrane components and provided the probes for the cDNA cloning of entactin and the three chains of laminin. The F9 cells have been widely employed to examine the regulation of expression of the laminin and entactin genes.
A E Chung
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  The International journal of developmental biology     Volume:  37     ISSN:  0214-6282     ISO Abbreviation:  Int. J. Dev. Biol.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-07-09     Completed Date:  1993-07-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8917470     Medline TA:  Int J Dev Biol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  141-50     Citation Subset:  IM    
University of Pittsburgh, Department of Biological Sciences, PA 15260.
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MeSH Terms
Basement Membrane / chemistry,  metabolism
Laminin / chemistry*,  metabolism,  physiology
Membrane Glycoproteins / chemistry*,  metabolism,  physiology
Neoplasm Proteins / chemistry*,  metabolism,  physiology
Teratoma / chemistry*,  metabolism
Grant Support
Reg. No./Substance:
0/Laminin; 0/Membrane Glycoproteins; 0/Neoplasm Proteins; 0/nidogen

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