| Embryo transfer experiments and ovarian transplantation identify the ovary as the only site in which nuclear receptor interacting protein 1/RIP140 action is crucial for female fertility. | |
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MedLine Citation:
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PMID: 11796527 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Spatial and temporal regulation of gene expression by a number of different nuclear receptors is critical in female reproduction. In this study we investigated whether the nuclear receptor corepressor nuclear receptor interacting protein 1 (Nrip1)/RIP140, which is essential for ovulation, is also required for postovulatory events, leading to pregnancy and parturition. Expression analysis indicated that Nrip1 is present in the uterus in stromal and glandular epithelial cells, primary decidual cells, and subsequently in differentiating decidual cells at the anti-mesometrial side of the implantation site. It also indicated a temporal regulation of Nrip1 in the corpora lutea at different stages of pregnancy, with increased levels at midgestation at approximately d 9.5 postcoitum (pc). By performing both embryo and ovarian transfer experiments we demonstrate that, provided the block to ovulation is by-passed, Nrip1(-/-) mice are capable of establishing and maintaining pregnancies. However, although the majority of offspring derived from ovarian transplantation survived, approximately 50% of embryos were resorbed by d 13.5 pc after embryo transfer, and the majority of pups were stillborn or died soon thereafter. Thus, although Nrip1 is differentially expressed in the reproductive tract, we conclude that the ovary is the only site in which its action is essential for fertility, with a crucial role in ovulation and a secondary role in the maintenance of pregnancy. |
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Authors:
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Göran Leonardsson; Mary Ann Jacobs; Roger White; Rosemary Jeffery; Richard Poulsom; Stuart Milligan; Malcolm Parker |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endocrinology Volume: 143 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2002 Feb |
Date Detail:
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Created Date: 2002-01-17 Completed Date: 2002-02-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 700-7 Citation Subset: AIM; IM |
Affiliation:
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Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College, London W12 1ONN, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing Animals Anovulation / genetics Embryo Transfer* Female Fertility / physiology* Galactosidases / biosynthesis, genetics Gene Expression Regulation, Enzymologic / genetics Genetic Markers In Situ Hybridization Infertility / genetics, pathology Mice Mice, Knockout Nuclear Proteins / genetics, physiology* Ovary / pathology, physiology*, transplantation* Pregnancy Progesterone / blood Receptors, Estrogen / genetics, physiology* Reverse Transcriptase Polymerase Chain Reaction Superovulation Uterus / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/Genetic Markers; 0/Nuclear Proteins; 0/Receptors, Estrogen; 0/nuclear receptor interacting protein 1; 57-83-0/Progesterone; EC 3.2.1.-/Galactosidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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