| Embryo production and endocrine response in ewes superovulated with PMSG, with or without monoclonal anti-PMSG administered at different times. | |
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MedLine Citation:
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PMID: 16727767 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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Mature nonlactating Altamurana ewes (n = 168) were synchronized in the seasonal anestrus period with FGA-impregnated intravaginal pessaries for 12 d. In Experiment 1, 48 ewes were divided into a 3 x 4 factorial design for anti-PMSG monoclonal antibody (AP) bioassay test. Concomitant injections of PMSG (1000, 1500, 2000 IU) and AP (0, 1, 2, 3 microl/IU PMSG) were given, and ovarian response was evaluated by laparoscopy. In Experiment 2, 120 ewes were divided into 8 experimental groups (n = 15 per group). The ewes treated with 1000 or 1500 IU PMSG at -24 h from sponge removal were given AP intravenously at 50 h after pessary withdrawal, 12 or 24 h after the onset of estrus, while the controls did not receive AP. Blood samples were collected from ewes (n = 6) treated with 1500 IU PMSG with or without anti-PMSG. Ovarian response and embryo production were evaluated on Day 7 after sponge removal upon laparotomy. It was found that 1 microl AP was effective in neutralizing 1 IU PMSG. No significant differences in serum concentrations of progesterone were observed among the groups of superovulated ewes. Estradiol-17 beta levels were reduced following AP treatment 12 h after the onset of estrus. At a lower dosage of superovulatory treatment (1000 IU PMSG), AP injected at 12 or 24 h after the onset of estrus significantly lowered large follicles (P < 0.01) and increased the rate of ovulation (P < 0.05). Moreover, embryo production showed a more than two-fold increase (P < 0.01) of viable embryos following AP injection at 12 or 24 h after the onset of estrus (3.2 to 3.3 vs 1.3, with vs without anti-PMSG). It is concluded that superovulatory treatment with 1000 IU PMSG plus AP administered at a fixed time after the onset of estrus may improve ovarian response and the yield of viable embryos in ewes. |
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Authors:
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G Martemucci; A D'Alessandro; F Toteda; A M Facciolongo; M Gambacorta |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Theriogenology Volume: 44 ISSN: 0093-691X ISO Abbreviation: Theriogenology Publication Date: 1995 Oct |
Date Detail:
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Created Date: 2006-05-26 Completed Date: 2007-07-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: United States |
Other Details:
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Languages: eng Pagination: 691-703 Citation Subset: - |
Affiliation:
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Dipartimento di Produzione Animale, Università degli Studi di Bari, Via G. Amendola, 165/A 70126 Bari, Italia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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