Document Detail


Embryo Sac Development in the Maize indeterminate gametophyte1 Mutant: Abnormal Nuclear Behavior and Defective Microtubule Organization.
MedLine Citation:
PMID:  12239418     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The indeterminate gametophyte1 mutation in maize has been known to disrupt development of the female gametophyte. Mutant embryo sacs have abnormal numbers and behavior of micropylar and central cell nuclei, which result in polyembryony and elevated ploidy levels in the endosperm of developing kernels. In this study, we confirm abnormal nuclear behavior and present novel findings. In contrast to the normal form, there is no obvious polarity in two-nucleate embryo sacs or in the micropylar cells of eight-nucleate embryo sacs. We show that the second and third mitoses are not fully synchronized and that additional mitoses can occur in all of the nuclei of the mutant embryo sac or in just the micropylar or central regions. After cellularization, individual micropylar cells can undergo mitosis. Abnormal microtubular behavior results in irregular positioning of the nuclei, asynchronous microtubular patterns in different pairs of nuclei, and abnormal phragmoplasts after the third mitotic division. These results indicate that in addition to acting primarily in controlling nuclear divisions, the indeterminate gametophyte1 gene acts secondarily in regulating microtubule behavior. This cytoskeletal activity most likely controls the polarization and nuclear migration underlying the formation and fate of the cells of the normal embryo sac.
Authors:
B. Q. Huang; W. F. Sheridan
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  The Plant cell     Volume:  8     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  1996 Aug 
Date Detail:
Created Date:  2002-Sep-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1391-1407     Citation Subset:  -    
Affiliation:
Department of Biology, University of North Dakota, Grand Forks, North Dakota 58202-9019.
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