| Embolization of a common carotid aneurysm with rhVEGF coupled to a pH-responsive chitosan in a rat model. | |
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MedLine Citation:
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PMID: 19698048 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECT: Treatment of cerebral aneurysms by endovascular deployment of liquid embolic agents has been proposed as an alternative strategy to conventional coiling, and new materials are being developed for embolization. In this study, the authors used a single-injection, biocompatible, biodegradable and pH-responsive acrylated chitosan (aCHN) with conjugated vascular endothelial growth factor (rhVEGF) in a rat aneurysm model. METHODS: The efficacy of the aCHN formulation with rhVEGF was tested using a common carotid artery occlusion model in rats, and the extent of embolization was evaluated using quantitative, qualitative, and histopathological techniques after 14 days of implantation. RESULTS: The mean occlusion was significantly greater for the rhVEGF/aCHN-treated group (96.8 +/- 3.0%) than for the group receiving aCHN (74.7 +/- 5.6%) (p < 0.01). Through qualitative evaluation, intimal and medial proliferation were significantly greater with rhVEGF/aCHN than with aCHN and controls (p < 0.001). Degradation of the aCHN filler was monitored in concert with the production of extracellular matrix components. Macrophages migrated in and proliferated inside the occluded carotid artery lumens were identified by histological and immunostainings. Results showed resorption of chitosan with concurrent development of collagen and elastin into the vessel lumen, suggesting clot maturation into fibrosis. CONCLUSIONS: Chitosan with a bioactive agent such as rhVEGF showed excellent results in occluding aneurysms in a rat model. |
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Authors:
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Hui Pan; Thomas Zimmerman; Julia Zakhaleva; John M Abrahams; Hongliang Jiang; Weiliam Chen |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of neurosurgery Volume: 112 ISSN: 1933-0693 ISO Abbreviation: J. Neurosurg. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-02 Completed Date: 2010-04-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0253357 Medline TA: J Neurosurg Country: United States |
Other Details:
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Languages: eng Pagination: 658-65 Citation Subset: AIM; IM |
Affiliation:
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Department of Biomedical Engineering, State University of New York-Stony Brook, Stony Brook, New York 11794-8181, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials / therapeutic use Brain / pathology, physiopathology Carotid Artery Diseases / pathology, physiopathology, therapy* Carotid Artery, Common* / pathology, physiopathology Chitosan / therapeutic use Collagen / metabolism Disease Models, Animal Elastin / metabolism Embolization, Therapeutic / methods* Extracellular Matrix / metabolism Fibrosis / pathology, physiopathology Hydrogen-Ion Concentration Intracranial Aneurysm / pathology, physiopathology, therapy* Macrophages / pathology, physiology Mitogens / therapeutic use Rats Rats, Sprague-Dawley Vascular Endothelial Growth Factor A / therapeutic use |
| Grant Support | |
ID/Acronym/Agency:
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DK068401/DK/NIDDK NIH HHS; HL088840/HL/NHLBI NIH HHS; NS059250/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Mitogens; 0/Vascular Endothelial Growth Factor A; 9007-34-5/Collagen; 9007-58-3/Elastin; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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