Document Detail

Embolization of a common carotid aneurysm with rhVEGF coupled to a pH-responsive chitosan in a rat model.
MedLine Citation:
PMID:  19698048     Owner:  NLM     Status:  MEDLINE    
OBJECT: Treatment of cerebral aneurysms by endovascular deployment of liquid embolic agents has been proposed as an alternative strategy to conventional coiling, and new materials are being developed for embolization. In this study, the authors used a single-injection, biocompatible, biodegradable and pH-responsive acrylated chitosan (aCHN) with conjugated vascular endothelial growth factor (rhVEGF) in a rat aneurysm model. METHODS: The efficacy of the aCHN formulation with rhVEGF was tested using a common carotid artery occlusion model in rats, and the extent of embolization was evaluated using quantitative, qualitative, and histopathological techniques after 14 days of implantation. RESULTS: The mean occlusion was significantly greater for the rhVEGF/aCHN-treated group (96.8 +/- 3.0%) than for the group receiving aCHN (74.7 +/- 5.6%) (p < 0.01). Through qualitative evaluation, intimal and medial proliferation were significantly greater with rhVEGF/aCHN than with aCHN and controls (p < 0.001). Degradation of the aCHN filler was monitored in concert with the production of extracellular matrix components. Macrophages migrated in and proliferated inside the occluded carotid artery lumens were identified by histological and immunostainings. Results showed resorption of chitosan with concurrent development of collagen and elastin into the vessel lumen, suggesting clot maturation into fibrosis. CONCLUSIONS: Chitosan with a bioactive agent such as rhVEGF showed excellent results in occluding aneurysms in a rat model.
Hui Pan; Thomas Zimmerman; Julia Zakhaleva; John M Abrahams; Hongliang Jiang; Weiliam Chen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of neurosurgery     Volume:  112     ISSN:  1933-0693     ISO Abbreviation:  J. Neurosurg.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-02     Completed Date:  2010-04-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0253357     Medline TA:  J Neurosurg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  658-65     Citation Subset:  AIM; IM    
Department of Biomedical Engineering, State University of New York-Stony Brook, Stony Brook, New York 11794-8181, USA.
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MeSH Terms
Biocompatible Materials / therapeutic use
Brain / pathology,  physiopathology
Carotid Artery Diseases / pathology,  physiopathology,  therapy*
Carotid Artery, Common* / pathology,  physiopathology
Chitosan / therapeutic use
Collagen / metabolism
Disease Models, Animal
Elastin / metabolism
Embolization, Therapeutic / methods*
Extracellular Matrix / metabolism
Fibrosis / pathology,  physiopathology
Hydrogen-Ion Concentration
Intracranial Aneurysm / pathology,  physiopathology,  therapy*
Macrophages / pathology,  physiology
Mitogens / therapeutic use
Rats, Sprague-Dawley
Vascular Endothelial Growth Factor A / therapeutic use
Grant Support
Reg. No./Substance:
0/Biocompatible Materials; 0/Mitogens; 0/Vascular Endothelial Growth Factor A; 9007-34-5/Collagen; 9007-58-3/Elastin; 9012-76-4/Chitosan

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