Document Detail


Embolic middle cerebral artery occlusion model using thrombin and fibrinogen composed clots in rat.
MedLine Citation:
PMID:  22985597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ischemic stroke accounts for over 80% in total human stroke which mostly affect middle cerebral artery (MCA) territory. Embolic stroke models induced by injection of homologous clots into the internal carotid artery and MCA closely mimic human stroke and have been commonly used in stroke research. Studies indicate that the size and composition of clots are critical for the reproducibility of the stroke model. In the present study, we modified the homologous clots formation by addition of thrombin and fibrinogen which produced even distribution of fibrin with tight cross linkage of red blood cells. We optimized the embolic MCA occlusion model in rats using different size of the mixed clots. A precise lodgment of the clots at the MCA bifurcation and highly reproducible ischemic lesion in the MCA territory were demonstrated in the embolic MCA occlusion model induced by injection of 10 pieces of 1-mm long mixed clots made in PE-60 catheter. We further tested the effect of recombinant tissue plasminogen activator (rtPA) in this embolic MCA occlusion model. rtPA induced thrombolysis, improved neurological outcome, and significantly reduced ischemic lesion volume when administered at 1h after embolism as compared with control. In summary, we have established a reproducible embolic MCA occlusion model using clots made of homologous blood, thrombin and fibrinogen. The mixed clots enable precise lodgment at the MCA bifurcation which is responsive to thrombolytic therapy of rtPA.
Authors:
Ming Ren; Zi-Jing Lin; Hai Qian; Gourav Roy Choudhury; Ran Liu; Hanli Liu; Shao-Hua Yang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-09-14
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  211     ISSN:  1872-678X     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-06     Completed Date:  2013-06-26     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  296-304     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anticoagulants / pharmacology
Disease Models, Animal*
Fibrinogen*
Infarction, Middle Cerebral Artery* / drug therapy,  pathology
Male
Rats
Rats, Sprague-Dawley
Thrombin*
Thrombosis*
Tissue Plasminogen Activator / pharmacology
Grant Support
ID/Acronym/Agency:
R01 NS054651/NS/NINDS NIH HHS; R01 NS054687/NS/NINDS NIH HHS; R01NS054651/NS/NINDS NIH HHS; R01NS054687/NS/NINDS NIH HHS; R21 NS052850/NS/NINDS NIH HHS; R21/R33NS052850/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Anticoagulants; 9001-32-5/Fibrinogen; EC 3.4.21.5/Thrombin; EC 3.4.21.68/Tissue Plasminogen Activator
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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