| EMBOLIC MIDDLE CEREBRAL ARTERY OCCLUSION MODEL USING THROMBIN AND FIBRINOGEN COMPOSED CLOTS IN RAT. | |
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MedLine Citation:
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PMID: 22985597 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Ischemic stroke accounts for over 80% in total human stroke which mostly affect middle cerebral artery (MCA) territory. Embolic stroke models induced by injection of homologous clots into the internal carotid artery and MCA closely mimic human stroke and have been commonly used in stroke research. Studies indicate that the size and composition of clots are critical for the reproducibility of the stroke model. In the present study, we modified the homologous clots formation by addition of thrombin and fibrinogen which produced even distribution of fibrin with tight cross linkage of red blood cells. We optimized the embolic MCA occlusion model in rats using different size of the mixed clots. A precise lodgment of the clots at the MCA bifurcation and highly reproducible ischemic lesion in the MCA territory were demonstrated in the embolic MCA occlusion model induced by injection of 10 pieces of 1-mm long mixed clots made in PE-60 catheter. We further tested the effect of recombinant tissue plasminogen activator (rtPA) in this embolic MCA occlusion model. rtPA induced thrombolysis, improved neurological outcome, and significantly reduced ischemic lesion volume when administered at 1hour after embolism as compared with control. In summary, we have established a reproducible embolic MCA occlusion model using clots made of homologous blood, thrombin and fibrinogen. The mixed clots enable precise lodgment at the MCA bifurcation which is responsive to thrombolytic therapy of rtPA. |
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Authors:
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Ming Ren; Zi-Jing Lin; Hai Qian; Choudhury Roy Gourav; Ran Liu; Hanli Liu; Shao-Hua Yang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-14 |
Journal Detail:
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Title: Journal of neuroscience methods Volume: - ISSN: 1872-678X ISO Abbreviation: J. Neurosci. Methods Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier B.V. |
Affiliation:
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Department of Pharmacology & Neuroscience, Institute for Aging and Alzheimer's Disease Research, University of North Texas Health Science Center, Fort Worth, 76107, TX, USA; Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing, 100093, P. R. China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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