Document Detail


Eluted rheumatoid synovial tissue T cell subsets and HLA-DR bearing cells at different stages of fractionation.
MedLine Citation:
PMID:  6236015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By using monoclonal antibodies of the OK series in the indirect immunofluorescence technique we wanted to enumerate T cell subsets and HLA-DR-bearing cells of patients with classical rheumatoid arthritis after the cells were eluted from rheumatoid synovial tissue and at different stages of two cell fractionation procedures. The procedures were an overnight incubation on plastic flasks or a brief (7 min) fractionation on nylon wool columns, both followed by Isopaque-Ficoll gradient centrifugation. 70-90% HLA-DR+ cells and 15-30% T3+ (T cells) were initially observed. Plastic flask incubation and gradient centrifugation reduced the mediam number of HLA-DR+ cells to 55% while the number of T3+ cells increased to 60-75%, the number of T4+ (helper/inducer T cells) and T8+ (cytotoxic/suppressor T cells) usually being about equal. The other fractionation procedure left essentially the same relative proportions of cells bearing these markers even though the cell yield was 5-10 times greater than for the plastic flask fractionation. About 30% of the T cells were calculated to bear HLA-DR antigens, indicating local activation in vivo.
Authors:
T Egeland; O Førre
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical rheumatology     Volume:  2     ISSN:  0770-3198     ISO Abbreviation:  Clin. Rheumatol.     Publication Date:  1983 Mar 
Date Detail:
Created Date:  1984-10-02     Completed Date:  1984-10-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8211469     Medline TA:  Clin Rheumatol     Country:  BELGIUM    
Other Details:
Languages:  eng     Pagination:  19-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / analysis
Arthritis, Rheumatoid / immunology*
Cell Separation / methods
HLA-DR Antigens
Histocompatibility Antigens Class II / analysis*
Humans
Leukocyte Count
Synovial Membrane / immunology*
T-Lymphocytes / immunology*
T-Lymphocytes, Cytotoxic / immunology
T-Lymphocytes, Helper-Inducer / immunology
T-Lymphocytes, Regulatory / immunology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/HLA-DR Antigens; 0/Histocompatibility Antigens Class II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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