| Eluted rheumatoid synovial tissue T cell subsets and HLA-DR bearing cells at different stages of fractionation. | |
| | |
MedLine Citation:
|
PMID: 6236015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
By using monoclonal antibodies of the OK series in the indirect immunofluorescence technique we wanted to enumerate T cell subsets and HLA-DR-bearing cells of patients with classical rheumatoid arthritis after the cells were eluted from rheumatoid synovial tissue and at different stages of two cell fractionation procedures. The procedures were an overnight incubation on plastic flasks or a brief (7 min) fractionation on nylon wool columns, both followed by Isopaque-Ficoll gradient centrifugation. 70-90% HLA-DR+ cells and 15-30% T3+ (T cells) were initially observed. Plastic flask incubation and gradient centrifugation reduced the mediam number of HLA-DR+ cells to 55% while the number of T3+ cells increased to 60-75%, the number of T4+ (helper/inducer T cells) and T8+ (cytotoxic/suppressor T cells) usually being about equal. The other fractionation procedure left essentially the same relative proportions of cells bearing these markers even though the cell yield was 5-10 times greater than for the plastic flask fractionation. About 30% of the T cells were calculated to bear HLA-DR antigens, indicating local activation in vivo. |
| | |
Authors:
|
T Egeland; O Førre |
Related Documents
:
|
9112215 - Immunocytochemical detection of breast cancer cells: a comparison of three attachment f... 7015075 - A method for electron microscopic preparation of cultured cells (monolayer) in a new te... 787425 - Quantitative immunoautoradiography at the cellular level. ii. absolute measurements usi... 3957455 - Experimental considerations for the centrifugal separation of blood cell components. 10821645 - Macrocyclic chelators with paramagnetic cations are internalized into mammalian cells v... 22528965 - Electrophysiological, morphological, and topological properties of two histochemically ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Clinical rheumatology Volume: 2 ISSN: 0770-3198 ISO Abbreviation: Clin. Rheumatol. Publication Date: 1983 Mar |
Date Detail:
|
Created Date: 1984-10-02 Completed Date: 1984-10-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8211469 Medline TA: Clin Rheumatol Country: BELGIUM |
Other Details:
|
Languages: eng Pagination: 19-26 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antibodies, Monoclonal
/
analysis Arthritis, Rheumatoid / immunology* Cell Separation / methods HLA-DR Antigens Histocompatibility Antigens Class II / analysis* Humans Leukocyte Count Synovial Membrane / immunology* T-Lymphocytes / immunology* T-Lymphocytes, Cytotoxic / immunology T-Lymphocytes, Helper-Inducer / immunology T-Lymphocytes, Regulatory / immunology |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/HLA-DR Antigens; 0/Histocompatibility Antigens Class II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effect of low doses of prednisolone on the traffic of T helper-inducer cells in rheumatoid arthr...
Next Document: Myelography--a case for audit.