Document Detail


Elucidation of peptide metabolism by on-line immunoaffinity liquid chromatography mass spectrometry.
MedLine Citation:
PMID:  10669885     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An immunoaffinity chromatography extraction capillary liquid chromatography separation has been coupled to electrospray ionization mass spectrometry for on-line characterization of drug metabolites of a therapeutic peptide in plasma. It is demonstrated that the selectivity, sensitivity and molecular weight data provided by immunoaffinity chromatography coupled to liquid chromatography/mass spectrometry provides a means of rapidly achieving qualitative determinations of small amounts of material in complicated biological matrices such as plasma. The ability to detect the peptide in rat plasma at a level of 10 ng/mL is demonstrated using this method. In addition, experiments to study the epitope of the peptide by enzymatic digestion and mass spectrometry are also discussed. The method is proposed as an alternative approach to studying the metabolism of therapeutic peptides.
Authors:
K Zheng; D M Lubman; D T Rossi; G D Nordblom; C M Barksdale
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  14     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2000  
Date Detail:
Created Date:  2000-03-20     Completed Date:  2000-03-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  261-9     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Ltd.
Affiliation:
Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies
Blood Chemical Analysis / instrumentation,  methods*
Cattle
Chromatography, Affinity / instrumentation,  methods*
Epitopes / blood,  chemistry
Evaluation Studies as Topic
Male
Mass Spectrometry / instrumentation,  methods*
Molecular Sequence Data
Online Systems
Peptides / blood*,  chemistry*,  immunology
Rabbits
Rats
Rats, Wistar
omega-Conotoxins / blood,  chemistry,  immunology
Grant Support
ID/Acronym/Agency:
R01 GM49500/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Epitopes; 0/Peptides; 0/omega-Conotoxins; 107452-89-1/ziconotide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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