| Elucidation of the gating of the GIRK channel using a spectroscopic approach. | |
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MedLine Citation:
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PMID: 19752111 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The traditional view of G protein-coupled receptor (GPCR)-mediated signalling puts the players in this signalling cascade, namely the GPCR, the G protein and its effector, as individual components in space, where the signalling specificity is obtained mainly by the interaction of the GPCR and the Galpha subunits of the G protein. A question is then raised as to how fidelity in receptor signalling is achieved, given that many systems use the same components of the G protein signalling machinery. One possible mechanism for obtaining the specific flow of the downstream signals, from the activated G protein to its specific effector target, in a timely manner, is compartmentalization, a spatial arrangement of the complex in a rather restricted space. Here we review our recent findings related to these issues, using the G protein-coupled potassium channel (GIRK) as a model effector and fluorescence-based approaches to reveal how the signalling complex is arranged and how the G protein exerts its action to activate the GIRK channel in intact cells. |
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Authors:
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Adi Raveh; Inbal Riven; Eitan Reuveny |
Publication Detail:
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Type: Journal Article; Review Date: 2009-09-14 |
Journal Detail:
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Title: The Journal of physiology Volume: 587 ISSN: 1469-7793 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-16 Completed Date: 2010-07-26 Revised Date: 2010-11-22 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
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Languages: eng Pagination: 5331-5 Citation Subset: IM |
Affiliation:
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Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Fluorescence Resonance Energy Transfer / methods*, trends G Protein-Coupled Inwardly-Rectifying Potassium Channels / chemistry*, metabolism, physiology* Humans Ion Channel Gating / physiology* |
| Chemical | |
Reg. No./Substance:
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0/G Protein-Coupled Inwardly-Rectifying Potassium Channels |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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