| Elo1p-dependent carboxy-terminal elongation of C14:1Delta(9) to C16:1Delta(11) fatty acids in Saccharomyces cerevisiae. | |
| | |
MedLine Citation:
|
PMID: 10850979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Saccharomyces cerevisiae medium-chain acyl elongase (ELO1) mutants have previously been isolated in screens for fatty acid synthetase (FAS) mutants that fail to grow on myristic acid (C14:0)-supplemented media. Here we report that wild-type cells cultivated in myristoleic acid (C14:1Delta(9))-supplemented media synthesized a novel unsaturated fatty acid that was identified as C16:1Delta(11) fatty acid by gas chromatography-mass spectroscopy. Synthesis of C16:1Delta(11) was dependent on a functional ELO1 gene, indicating that Elo1p catalyzes carboxy-terminal elongation of unsaturated fatty acids (alpha-elongation). In wild-type cells, the C16:1Delta(11) elongation product accounted for approximately 12% of the total fatty acids. This increased to 18% in cells that lacked a functional acyl chain desaturase (ole1Delta mutants) and hence were fully dependent on uptake and elongation of C14:1. The observation that ole1Delta mutant cells grew almost like wild type on medium supplemented with C14:1 indicated that uptake and elongation of unsaturated fatty acids were efficient. Interestingly, wild-type cells supplemented with either C14:1 or C16:1 fatty acids displayed dramatic alterations in their phospholipid composition, suggesting that the availability of acyl chains is a dominant determinant of the phospholipid class composition of cellular membranes. In particular, the relative content of the two major phospholipid classes, phosphatidylethanolamine and phosphatidylcholine, was strongly dependent on the chain length of the supplemented fatty acid. Moreover, analysis of the acyl chain composition of individual phospholipid classes in cells supplemented with C14:1 revealed that the relative degree of acyl chain saturation characteristic for each phospholipid class appeared to be conserved, despite the gross alteration in the cellular acyl chain pool. Comparison of the distribution of fatty acids that were taken up and elongated (C16:1Delta(11)) to those that were endogenously synthesized by fatty acid synthetase and then desaturated by Ole1p (C16:1Delta(9)) in individual phospholipid classes finally suggested the presence of two different pools of diacylglycerol species. These results will be discussed in terms of biosynthesis of different phospholipid classes via either the de novo or the Kennedy pathway. |
| | |
Authors:
|
R Schneiter; V Tatzer; G Gogg; E Leitner; S D Kohlwein |
Related Documents
:
|
3794719 - Mechanism of arachidonic acid liberation during ischemia in gerbil cerebral cortex. 22170289 - Combined muta- and semisynthesis: a powerful synthetic hybrid approach to access target... 3139039 - Effects of dietary butter enrichment on the fatty acid distribution of phospholipid fra... 7150339 - Evidence for a carbachol stimulated phosphatidylinositol effect in heart. 325229 - Effects of temperature and host cell genetic characteristics on the replication of the ... 8063229 - Study of plasma and red cell phospholipid fatty acids in extrahepatic cholestatic jaund... 3058829 - Filamentous phase in life cycle of m. leprae(?): a preliminary communication. 10737359 - Bile acid secretion during rat liver carcinogenesis. 10521359 - Eicosapentaenoic acid and docosahexaenoic acid block serotonin-induced smooth muscle ce... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of bacteriology Volume: 182 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 2000 Jul |
Date Detail:
|
Created Date: 2000-08-15 Completed Date: 2000-08-15 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 3655-60 Citation Subset: IM |
Affiliation:
|
Institut für Biochemie und Lebensmittelchemie, Technische Universität Graz, A-8010 Graz, Austria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetyltransferases
/
metabolism* Culture Media Fatty Acids, Monounsaturated / metabolism* Fatty Acids, Unsaturated / biosynthesis* Phospholipids / metabolism Saccharomyces cerevisiae / enzymology*, genetics, growth & development Saccharomyces cerevisiae Proteins* |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 0/Fatty Acids, Monounsaturated; 0/Fatty Acids, Unsaturated; 0/Phospholipids; 0/Saccharomyces cerevisiae Proteins; 544-64-9/9-tetradecenoic acid; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/ELO1 protein, S cerevisiae; EC 2.3.1.-/fatty acid elongases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characterization of the fructosyltransferase gene of Actinomyces naeslundii WVU45.
Next Document: Purification and characterization of Sa-lrp, a DNA-binding protein from the extreme thermoacidophili...