Document Detail

Ellagic acid, phenolic acids, and flavonoids in Malaysian honey extracts demonstrate in vitro anti-inflammatory activity.
MedLine Citation:
PMID:  20934607     Owner:  NLM     Status:  MEDLINE    
Natural honey has been used in traditional medicine of different cultures throughout the world. This study looked into the extraction of Malaysian honey and the evaluation of the anti-inflammatory activity of these extracts. It was hypothesized that honey extracts contain varying amounts of phenolic compounds and that they possess different in vitro anti-inflammatory activities. Honey extracts were analyzed using liquid chromatography-mass spectrometry to identify and compare phenolic compounds, whereas high-performance liquid chromatography was used for their quantification. Subsequently, honey methanol extract (HME) and honey ethyl acetate extract (HEAE) were tested in vitro for their effect on nitric oxide production in stimulated macrophages. The extracts were also tested for their effects on tumor necrosis factor-α (TNF) cytotoxicity in L929 cells. The major phenolics in the extracts were ellagic, gallic, and ferulic acids; myricetin; chlorogenic acid; and caffeic acid. Other compounds found in lower concentrations were hesperetin, p-coumaric acid, chrysin, quercetin, luteolin, and kaempferol. Ellagic acid was the most abundant of the phenolic compounds recorded, with mean concentrations of 3295.83 and 626.74 μg/100 g of honey in HME and HEAE, respectively. The median maximal effective concentrations for in vitro nitric oxide inhibition by HEAE and HME were calculated to be 37.5 and 271.7 μg/mL, respectively. The median maximal effective concentrations for protection from TNF cytotoxicity by HEAE and HME were 168.1 and 235.4 μg/mL, respectively. In conclusion, HEAE exhibited greater activity in vitro, whereas HME contained a higher concentration of phenolic compounds per 100 g of honey.
Mustafa Kassim; Mouna Achoui; Mohd Rais Mustafa; Mustafa Ali Mohd; Kamaruddin Mohd Yusoff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nutrition research (New York, N.Y.)     Volume:  30     ISSN:  1879-0739     ISO Abbreviation:  Nutr Res     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-10-11     Completed Date:  2011-01-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8303331     Medline TA:  Nutr Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  650-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Department of Anesthesiology, University of Malaya, 50603 Kuala Lumpur, Malaysia.
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MeSH Terms
Anti-Inflammatory Agents / analysis,  pharmacology*
Biological Products / chemistry,  pharmacology*
Cell Line
Cell Line, Tumor
Ellagic Acid / analysis,  pharmacology*
Flavonoids / analysis,  pharmacology*
Macrophages / drug effects*
Nitric Oxide / antagonists & inhibitors*
Phenols / analysis,  pharmacology*
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Biological Products; 0/Flavonoids; 0/Phenols; 0/polyphenols; 10102-43-9/Nitric Oxide; 476-66-4/Ellagic Acid

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