Document Detail


Ellagic acid inhibits lipopolysaccharide-induced expression of enzymes involved in the synthesis of prostaglandin E2 in human monocytes.
MedLine Citation:
PMID:  19948080     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ellagic acid, a natural polyphenol found in certain fruits, nuts and vegetables, has in recent years been the subject of intense research within the fields of cancer and inflammation. Pain, fever and swelling, all typical symptoms of inflammation, are ascribed to elevated levels of PGE2. In the present study, we have investigated the effects of ellagic acid on PGE2 release and on prostaglandin-synthesising enzymes in human monocytes. Ellagic acid was found to inhibit Ca ionophore A23187-, phorbol myristate acetate- and opsonised zymosan-induced release of PGE2 from monocytes pre-treated with the inflammatory agent lipopolysaccharide. Ellagic acid suppressed the lipopolysaccharide-induced increase in protein expression of cyclo-oxygenase-2 (COX-2), microsomal PGE synthase-1 (mPGEs-1) and cytosolic phospholipase A2alpha (cPLA2alpha), while it had no effect on the constitutively expressed COX-1 protein. Ellagic acid had no apparent inhibitory effect on these enzymes when the activities were determined in cell-free assays. We conclude that the inhibitory effect of ellagic acid on PGE2 release from monocytes is due to a suppressed expression of COX-2, mPGEs-1 and cPLA2alpha, rather than a direct effect on the activities of these enzymes.
Authors:
Sofia Karlsson; Eewa N?nberg; Christina Fjaeraa; Jonny Wijkander
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-01
Journal Detail:
Title:  The British journal of nutrition     Volume:  103     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-13     Completed Date:  2010-04-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  1102-9     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biomedical Sciences, Karlstad University, Karlstad, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Calcimycin / antagonists & inhibitors
Cyclooxygenase 2 / metabolism
Dinoprostone / blood,  secretion*
Ellagic Acid / pharmacology*
Humans
Intramolecular Oxidoreductases / metabolism
Kinetics
Lipopolysaccharides / antagonists & inhibitors,  pharmacology*
Microsomes / enzymology
Monocytes / drug effects,  enzymology*
Phospholipases A2 / metabolism
Subcellular Fractions / drug effects,  enzymology
Tetradecanoylphorbol Acetate / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Lipopolysaccharides; 16561-29-8/Tetradecanoylphorbol Acetate; 363-24-6/Dinoprostone; 476-66-4/Ellagic Acid; 52665-69-7/Calcimycin; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 3.1.1.4/Phospholipases A2; EC 5.3.-/Intramolecular Oxidoreductases; EC 5.3.99.3/prostaglandin-E synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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