Document Detail


Elimination of porcine heptadecapeptide gastrin (G17) and human leu big gastrin (G34) by the perfused pig liver.
MedLine Citation:
PMID:  6745510     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to study some of the molecular events during the hepatic passage of gastrin, we perfused sulfated natural porcine gastrin (G17 II) through isolated pig livers. The disappearance half time of G17 II was about 20-30 min when the starting gastrin concentrations were greater than 100 pM; lower concentrations were reduced with half times of 40-100 min. Synthetic human leu-32 (G34) was not eliminated. The use of region-specific antibodies to gastrin indicated that degradation was more effective at the N-terminus of gastrin. Whereas Sephadex chromatography revealed no change of the molecular size, SDS-polyacrylamide gel electrophoresis showed the presence of smaller immunoreactive fragments of gastrin in addition to immunoreactive fragments of gastrin of the heptadecapeptide size. These findings indicate that the isolated porcine liver degrades porcine G17 to smaller fragments.
Authors:
G E Feurle; C Winkler; A Encke; V Helmstaedter; J G Doertenbach
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Digestion     Volume:  29     ISSN:  0012-2823     ISO Abbreviation:  Digestion     Publication Date:  1984  
Date Detail:
Created Date:  1984-08-30     Completed Date:  1984-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0150472     Medline TA:  Digestion     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  183-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Gastrins / metabolism*
Humans
Liver / metabolism*,  physiology
Protein Precursors*
Swine
Chemical
Reg. No./Substance:
0/Gastrins; 0/Protein Precursors; 53988-98-0/big gastrin; 60748-06-3/gastrin 17

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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